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科展作品檢索
CONTACTLESS AND NON-DESTRUCTIVE DETECTION OF CHICKEN MEAT CONTAMINATION WITH LASER SPECKLE METHOD
Harmful microorganisms in food can cause deterioration of human health, poisoning and in some cases even death. Especially fresh meat and chicken products create a suitable environment for the growth of microorganisms in terms of the nutrients it contains, water activity and pH level. For this reason, detection of microorganisms in meat products is an important issue in terms of food safety and human health. In this project, it is aimed to detect live microorganisms in meat products, especially chicken meat, in a simple, non-destructive, non-contact and fast way using laser speckle method. Laser speckle images of healthy and stale chicken meat were taken, contrast parameter and correlation analysis of the obtained patterns were made. It was observed that the contrast parameter for staled chicken meat increased by approximately 3 times compared to fresh chicken. This increase provides an understanding of the difference between contaminated chicken and fresh chicken. Speckle density changes over time in relation to the movements of living microorganisms. Thus, the correlation in laser speckle density patterns taken from contaminated tissues is disrupted. In the measurements taken with photodiode, by analyzing the change of light intensity of the speckle patterns on fresh and contaminated tissues over time, the detection of microorganisms was made easier and more precisely without the need for image processing. The proposed measurement system is a new method that detects meat contamination with laser speckle imaging. It can be developed and made portable and can be used easily in homes. Since it is a simple, non-destructive and fast method, it can be used to determine the shelf life of meat in food distribution places and markets. In addition, it has the potential to be calibrated and used for other food products other than meat products. The system developed with this study is cheap and easy to use, and the laser speckle imaging method is used in a different field other than biomedical, contributing to the literature.
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Prismalla: Mist water collector
The lack of drinking water in human settlements triggers a series of problems that are linked and affect the development of humanity: health problems, lack of water security for companies, lack of jobs, insecurity, among others. We observe this problem in the communities of the municipality of Las Vigas de Ramírez, Veracruz, where there is a great problem with the water supply, although there is a high presence of mist. Faced with this situation, we undertook the task of investigating a water harvesting method that is easy to implement, operate and maintain. We investigated and analyzed the methods of mist condensation through physical barriers, finding that the polyethylene shadow mesh was the means to achieve this, because it allows the passage of the wind, it is very light, easy to manipulate and above all that it presents the phenomenon of percolation that allows water droplets of various diameters to be accommodated therein. We designed a device that allows to present a mist catchment area through a prismatic structure enabled with meshes and condensed water receivers, portable, easy to use and maintenance and very economical with a performance of 20 liters per day. To achieve our project, factors such as air humidity, dew point, wind speed and direction, height, temperatures and available spaces must be considered.
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Emotional Stress Relief Vest – Research on Heat Dissipation
Due to having Asperger's syndrome, I want to make a pressure vest suitable for hot and humid climates like Asia to help reduce the daily anxiety of children and adults with autism, ADHD, sensory disorders, etc. To solve this problem, I first tried using a porous material. But after seeing a pressure vest in person, I found it to be useless. Therefore, I came up with the idea of creating an active airflow cooling system between the body and the pressure vest. To experiment, I wanted to make pressure vests. However, I found that a high-frequency TPU welding machine is required. Thus, I made models of pressure vests using airbags, water-resistant nylon, and quick-drying fabric. I also made models, which came from sandwich fabric. Through this research, I discovered that the sandwich fabric design has the best passive heat dissipation, and the combination of the sandwich fabric and the active airflow cooling system has the best active heat dissipation.
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激增殺手T細胞的水膠細胞平台於免疫療法的應用
本實驗研發新型水膠細胞優化技術,成功提供一個平台供應淋巴細胞激活信號(MHC-antigen complex)、共刺激配體及細胞因子刺激三大要素,能激增殺手T細胞。實驗中優化的水膠細胞能完整保留細胞膜的表面特性,經實驗證實無生物安全性的疑慮。另外本實驗也著重於探討人工合成抗原細胞強韌度對T細胞增生程度的影響。我們利用水膠細胞易改變細胞質強韌度且不影響生物相容性的優點,針對T細胞增生程度進行比較。本研究結果顯示,高細胞質強韌度(120kPa)的人工合成抗原細胞(aAPCs, Artificial antigen presenting cells)擁有高效率的T細胞激活潛力,相對於低細胞質強韌度(25kPa)的對照組可以提高約51.2%,並達到顯著差異,推測其和收縮性及突出性絲狀肌動蛋白增加以致訊號增強有關。本實驗的新型水膠細胞優化技術提供臨床醫學免疫療法一個高潛力的新技術平台。
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DEVELOPMENT AND USE OF LASER 3D SCANNER OF PREMISES
This research work is devoted to the stages of development and creation of a prototype of a laser 3D scanner model, programming of a controlling microcontroller, construction of 3D models of a scanned object. In the course of the work, the market of 3D scanners, which are used to build three-dimensional models of premises, was analyzed, the equipment necessary for the development and creation of the prototype was analyzed, as well as the software necessary for the operation of the prototype. The result of the work was the creation of a laser 3D scanner based on an Arduino microcontroller using a Lidar type sensor that scans and builds 3D models of objects. This working model of the 3D scanner demonstrates good capabilities and turned out to be easy to use.
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A New Method For Microplastic Removal and Optical Measurement
Microplastics are tiny invisible plastic pieces that are piling up in the marine environment emerging as one of the many environmental issues which our planet is facing today. Researches for the removal of these particles are important because studies that have been made so far haven't come up with an effective solution. This project aimed to detect microplastics and remove them from aqueous environments with an effective and practical method then it was aimed to determine the removal amount of microplastics by optical measurements with the developed system. Firstly, the magnetic carbonanotubes (m-CNT) which is intended to hold onto the surfaces of microplastics was synthesized and added to the mixture of microplastics. Then the magnet within a glass tube was passed through the mixture and the sample was cleared of microplastics. A spectrometer was made to monitor this process and after its calibration, it was used to measure coffees with different concentrations. It has been shown that their concentrations can be determined by calculating the transmission values and Rayleigh scattering. In the end, it has shown that there are no micro or nano-sized plastic particles when removed with M-CNT, within the accountable range of the spectrometer that had been made. Hence the removal of the microplastics: an invisible threat for the environment has been studied by combining nanomaterials with unique surface properties in the removal process and an optical principle such as Rayleigh scattering, a new technique has been developed that can measure quickly, economically,
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Cross-lingual Information Retrieval
In this project, we evaluate the effectiveness of Random Shuffling in the Cross Lingual Information Retrieval (CLIR) process. We extended the monolingual Word2Vec model to a multilingual one via the random shuffling process. We then evaluate the cross-lingual word embeddings (CLE) in terms of retrieving parallel sentences, whereby the query sentence is in a source language and the parallel sentence is in some targeted language. Our experiments on three language pairs showed that models trained on a randomly shuffled dataset outperforms randomly initialized word embeddings substantially despite its simplicity. We also explored Smart Shuffling, a more sophisticated CLIR technique which makes use of word alignment and bilingual dictionaries to guide the shuffling process, making preliminary comparisons between the two. Due to the complexity of the implementation and unavailability of open source codes, we defer experimental comparisons to future work.
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The development of natural quick-cooking tapioca balls
本研究利用自製包覆薄膜裝置,在濕粉圓表面包覆雙層海藻酸鈣薄膜(內層1.0%海藻酸鹽+外層0.5%海藻酸鹽),經自然乾燥製成新型粉圓,可浸泡冷水不會崩解或破裂,且水分完全滲入粉圓內部僅需25分鐘;新型粉圓在泡水25分鐘後,其內部水分含量高達51.67%。新型粉圓要達到100%煮熟率之時間,僅乾粉圓的37.80%、濕粉圓的42.75%,省時效果十分明顯;而所消耗電能,僅乾粉圓的25.57%、濕粉圓的28.81%,節能效果極為顯著。新型粉圓經全質構分析(TPA)之彈性已達到商品化之水準,並經官能品評驗證,受試者對新型粉圓各項目之喜好程度均優於濕粉圓。最後,測試新型粉圓之水活性僅為0.684,其Aw值明顯低於一般微生物生長界限0.8,可在常溫下長時間貯存。
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Laying waste to Energy problems
This research aims at exploiting civil and pre-treated industrial wastewaters that go into the purifier and those that come out of it after various treatments in order to build a galvanic cell with the goal of producing clean electric energy. Our background hypothesis is that it is possible to exploit the existing potential difference between these two types of water to generate electricity. In fact, the water sent for purification contains elements (carbon, nitrogen, sulphur, phosphorus, etc.) in a predominantly "reduced" state and its oxygen level is scarce. On the other hand, the water coming out of the process contains the same elements in a mostly "oxidized" state and it is rich in oxygen. Those chemical discrepancies should get the job done. In order to simulate the two types of water, two different solutions were prepared. The first one is highly concentrated with pollutants and gaseous nitrogen is insufflated in it to reproduce its anoxic environment. The second one’s pollution level is based on the Italian legislative limits of chemical contaminants for superficial waters (Legislative Decree 152/2006) and the semi-cell is insufflated with gaseous oxygen.
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BIOINFORMATIC PREDICTION OF CORONAVIRUS (SARS-COV-2) MUTATIONS THAT INCREASE CONTAGIOUSNESS
Inhibitory effects of the secondary metabolite of actinomycete were examined on cell cycle of the yeasts of S. pombe and S. cerevisiae. The secondary metabolite was obtained from cultivation of the actinomycete isolated from the soil of Owakudani in Hakone, Japan. The fifth fraction of the secondary metabolite by ODS column separation (HK-T5), which was soluble to pure methanol, was used in the present experiments. The HK-T5 brought about the delay of forming colonies of S. pombe for about 11 days compared to that cultivated without the HK-T5. The delay of the colony formation was longer for the S. pombe cultivated with more amount of the HK-T5. The cultivation with HK-T5 also brought about the extension of the lifespan of the S. pombe for more than 10 weeks in a liquidus medium. The cell life recovered the ordinary manner by removal of the HK-T5, meaning that the activities of the HK-T5 is reversible. These facts confirm the suppression of cell cycle, and the delay of cell growth by the HK-T5. These phenomena were similarly observed for S. cerevisiae. Comparison of the action of HK-T5 with hydroxyurea, which is an anticancer drug inhibiting the cell cycle at S phase, clarified that the inhibitory action of HK-T5 worked at the phase earlier than S phase. The combined effects of HK-T5 on the cell cycle were evaluated with triamcinolone acetonide (TA), or aspirin, the former of which is a drug synchronizing cancer cells in S phase, and the latter keeping human cells in G1/G0 phases. The combined use of HK-T5 with TA synchronized the cells at the phase slightly proceeding from G1 to S phase without toxicity. On the other hand, the combined use with aspirin made the inhibitory effect of HK-T5 inactive. Hence, the HK-T5 is attractive as a drug for the extension of cell lifespan, and anticancer therapy.
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近年河川或海上塑膠垃圾與油汙對環境造成傷害,解決環境污染備受重視。近期荷蘭用-大氣泡屏障,成功將塑膠垃圾阻攔,因此我們興起了「尋找最佳氣泡牆模型」。本研究目的為:分析「氣泡牆形狀、氣泡間隔以及氣泡大小」是否影響攔阻效果。結果發現:1.凹向水源300弧線氣泡牆攔阻效果最佳;2.氣泡越小間距越小,攔阻效果越好,此與荷蘭研究員論點相呼應;3.相較於形狀固定的塑膠垃圾,形狀會變的油汙較難攔阻;4.具厚度氣泡牆,攔阻效果更勝多道獨立的氣泡牆;5.「緊鄰的兩道300弧線氣泡牆」的攔阻效果是目前我們所做的模型中效果最好的。因此我們建議,未來在實際運用氣泡牆攔阻汙染源時,可採用此模式解決環境污染問題。
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Development of an autonomous Search and Rescue Drone
The number of natural disasters has risen significantly in recent years, and with climate change there is no end in sight. Consequently, the demands on rescue forces around the world are increasing. For this reason, I asked myself what I can do to improve the work of rescue teams. Advances in artificial intelligence and drone technology enable new possibilities for problem solving. Based on the technological advances mentioned above, an autonomous Search and Rescue drone was developed as part of this project. The system assists rescue workers in searching for survivors of natural disasters or missing people. This paper also suggests a method for prioritizing survivors based on their vitality. The system was implemented using a commercial Parrot ANAFI drone and Python. The software was tested on a simulated drone. To simplify the development, the whole system was divided into the following subsystems: Navigation System, Search System and Mission Abort System. These subsystems were tested independently. The testing of solutions and new concepts were performed using smaller test programs on the simulated drone and finally on the physical drone. The Search and Rescue system was successfully developed. The person detection system can detect humans and distinguish them from the environment. Furthermore, based on the movements of a person, the system can distinguish whether the person is a rescuer or a victim. In addition, an area to be flown over can be defined. If something goes wrong during the mission, the mission can be aborted by the Mission Abort System. In the simulation, the predefined area can successfully be flown over. Unfortunately, controlling the physical drone does not work. It stops in the air after takeoff due to the firmware of the drone. It does not change the flight state of the drone, which results in all subsequent commands from the system being ignored. This paper shows that artificial intelligence and drone technologies can be combined to deliver better rescue services. The same system can be applied to other applications.
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