We operated the misexpression screen between the EP lines and the pattern lines with the genotypes of eq1>dll, eq1>abdA, eq1>Ubx, eq1-GAL4, ey-GAL4 or dpp-GAL4. After the screening, we found that five of these 1,800 strains of filial generation had special phenotypes. It had shorter antennae and defects in the anterior equatorial region of eyes. We used plasmid rescue and IPCR to sequence the certain target gene, and found that it was escargot, abbreviated as esg. To identify when, where and how the overexpression of escargot induces such phenotype, we operated the staining of eye-antenna disc in third-instar larval period of wild type, eq> esg×UAS-GFP and eq>GFP with anti-dll, anti-caspase3 and anti-esg. The result shows that escargot cannot be detected before puparium formation. But the expression of dll, a gene controls the eye development, was reduced in the eye disc. We except the overexpression cause the defect of distal antennae and the anterior equatorial region of eyes mainly in the 3-day-long pupal life.我們用異位表現法篩選出和eq1>dll、eq1>abdA、eq1>Ubx、eq1-GAL4、ey-GAL4或dpp-GAL4 這些pattern lines有交互作用的EP lines。在這1800種的果蠅子代品系中，有五種具有特殊的性 狀。它們具有觸角短化以及複眼前緣中央區有缺刻的現象(形成心型眼)。我們使用質體救援 法以及IPCR的方法來定序這段未知基因序列，發現這是一個叫做escargot的基因(簡稱esg)。 為了了解過分表現此基因會造成何種分子影響，以至於產生此種性狀，因此我們使用 anti-dll 、anti-caspase3 和anti-esg 進行野生型、eq>esg×UAS-GFP 和eq>GFP 三齡幼蟲的 eye-antenna disc的螢光免疫染色。結果在幼蟲成蛹前都沒有偵測到esg的表現現象；不過在eye disc中，控制眼睛發育的基因dll的表現有被抑制的現象。因此我們推測過分表現esg的過程因 該是發生在為其短短三天的蛹期。也就是說，這種表型應該是在化蛹後形成。

週六的團體活動時問，老師會帶領班上同學玩一些益智的科學遊戲，讓我們動動腦。其中「繆比烏絲帶」最令班上同學感到訝異與著迷。有一天，老師帶我們到植物園的科學教育館參觀時，竟然又看到超大型的「繆比烏絲帶」模型，只可惜底下的說明圖並未進一步介紹剪開後的神奇變化，更激發了我們的興趣與好奇，因此，在老師的帶領下，我們運用系統化的方法，按部就班的仔細分析、探討，並記錄其結果，最後終於找出紙帶扭轉次數與剪開後的結果之間的關係了。並意外發現，封閉曲線交叉所產生的交叉點數與其所圍出的區域數之間的關係了。

選用常見之不同糖類製作膨糖，結果發現以二砂製出之成品，其風味、整體接受性方面，比較其他糖類表現為佳(p

耕土之肥沃度及作物生產量與土壤微生物實有不可分割的關係(Sadasivan 1970) (7) , Waksman ( 1922 )曾經主張以土壤微生物的數量來衡量土壤的肥沃力(8)。但至 n 前為止，微生物在土壤之生態及生理以及與高等作物營養供需的關係均缺乏了解，所以一般概念均認為由於微生物的表而積遠大於高等植物根部的表面積，故加入土壤中之肥料瞬間即被微生物使用殆盡，造成高等植物可用之營養素 (Available nutrients) 缺乏，而忽略了微生物乃是高等植物營養之儲藏及供應的來源（尤其固氮菌與固磷菌對於土壤中無氮磷循環與堆持，佔有極重要的角色，而對於土壤肥力的保持也有舉足輕重的地位），有其益於植物之一面也。

在警局學習指紋鑑定中了解，要從線索裡找出犯罪的指紋是艱辛的，所以本次實驗就五種檢測法配合染色，了解不同材質的狀況，加上警局最常用的寧海德林試劑做最佳化研究，實驗發現如下： 不同材料適合的方法 1、吸水性材料指紋顯像最適方法為寧海德林法。 2、非吸水性材料指紋顯像以龍膽紫法、粉末法及氰丙烯酸酯法最適合。 寧海德林試劑最佳化 1、指紋浸泡寧海德林試劑約兩分鐘效果最理想。 2、指紋樣本以60℃烘30分鐘，顯像最佳。 3、酸會破壞指紋，鹼會溶化一部分指紋，在pH=6時寧海德林顯像最佳。 4、配置原始寧海德林試劑需六次萃取，可以發現在四次以內效果仍佳。 5、發現濃度在原始寧海德林試劑的60%仍有不錯表現，可以依此做改良。

有一天上數學課的時候，老師忽然提出了一個問題：「用1元、s元、10元、50元和100元的錢幣湊成 1256元，有幾種湊法？」我們覺得這個問題很有趣，但是一時之問答不上來，課後三五成羣，聚在一起討論，拿出錢幣排排看，發現情形錯綜複錐、種類繫多，無從著手，於是想到分類整理，作圖解，第二天提出了許多不同的答案，…… 414種，102 種 ..九百多種，二千多種 · · · · · · · 一但都沒有 172 把攔，隨後老師告訴我們種敵很多，不只這些，要知道正確答案，須先簡化問題，列表，找出規律性，並告訴我們這種方法是以後做學問常用的方法。於是在老師的指導下，我們做了以下的研習活動。

高效液相層析儀（HPLC）為測定中藥指標成分常用的分析工具，而近年來高效液相層析質譜儀（LC-MS）的應用，亦在天然藥物分析上逐漸扮演重要角色。本研究利用HPLC 分析人參中的12 種人參皂素，並以 LC-MS 確認各化合物。 本研究分為三個部份，第一部份為開發適當的HPLC 分析方法分析人參中的主要成份，並使用LC-MS 確認這些化合物。人參中的12 種人參皂素( ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2, Ro and malonylginsenosides mRb1, mRb2 and mRc ) 可於60 分鐘內成功分離，在 HPLC 分析方法中使用Cosmosil 5C18 管柱，以200nm 偵測，並以20mM KH2PO4-CH3CN 進行梯度沖提， 在LC-MS 分析中則以20mM CH3COONH4-CH3CN 進行梯度沖提。 第二部份為利用上述分析方法，比較同批人參藥材進行不同處理方式後，所得人參皂素含量的多寡，包含泡茶、煮茶、泡酒等。結果顯示若為切段（飲片）泡茶，至少應浸泡30 分鐘，切段（飲片）水煮則以15-20 分鐘較佳，粉末（茶包）泡茶的變化不明顯，而切段酒浸可以得到最多量的人參皂素，且浸泡越久含量越高。 第三部份為使用多變量統計軟體SPSS 進行區別分析（DA），統計結果可作為人參藥材基原辨識之依據。 High-performance liquid chromatography (HPLC) is a common analytical tool that has been used to analyze the index components of Chinese herb medicine. In recent years, high-performance liquid chromatography-mass spectrometry (LC-MS) gradually plays an important role. In this study, we used HPLC to detect 12 ginsenosides in Ginseng Radix, and used LC-MS to identify the 12 compounds. This study includes three parts. The first part concerns the development of HPLC method for determining the chemical constituents in Ginseng Radix, and using LC-MS to identify them. In Ginseng Radix, components such as ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2, Ro and malonylginsenosides mRb1, mRb2 and mRc could be separated successfully in 60 mins. They were detected at 200nm by using a Cosmosil 5C18 column with a linear gradient elution of 20mM KH2PO4-CH3CN in the HPLC method, and with a linear gradient elution of 20mM CH3COONH4-CH3CN in the LC-MS method. In the second part, we used this method mentioned above to evaluate the different results from various processes, which include brewing snippets in hot water, brewing powders in hot water, cooking snippets with boiling water, and brewing snippets in rice wine. Our results are listed as follows, First, the tea using Ginseng Radix snippets brewing for about 30 mins in hot water has the best result. Second, using Ginseng Radix snippets cooking for about 15-20 mins with boiling water has the best result. Third, using Ginseng Radix powders brewing in hot water has no apparent difference. Finally, using Ginseng Radix snippets brewing in the rice wine can get most ginsenosides. The third part is using the statistic software SPSS to proceed the Discriminate Analysis（DA）. The results of the statistic analysis can help us to identify the origin of the herb medicines.

The purpose of this project is to determine if it is possible to use Ascorbic Acid Sodium- Dependent Vitamin C Transporter Type II, SVCT2, as an effective and safe protein to attach to certain brain tumor treatments to bypass the Blood Brain Barrier (BBB). Stemming from this problem, a procedure was created to use in vitro engineering with the aid of a professor at the University of Calgary to combine SVCT2 and three specific tumor treatments; Imatinib Mesylate (STI-571), Temsirolimus (CCI-779), and Suberoylanilide Hydroxamic Acid (SAHA). Following this, a metabolic barrier had to be created to simulate the BBB. To do this, the use of three enzymes were mixed and held together using specific bonds. Finally, a special bio-tracer was placed within the barrier to detect any toxic effects that may be produced. Then two trials were made with each treatment on the barrier at 34°C, 37°C, and 39°C. Once this was done observations could be made. When the newly isolated SVCT2 attaches to the three cancer treatments, they would all be able to connect and form bonds with each other. Once the incubation period is over for the first trial at 34°C, 37°C and 39°C, several things would be observed within the data. When counting the number of cells that were able to get into the engineered metabolic barrier, it could be seen that there was a dramatic increase in the number of cells in the 37°C range. SVCT2 can be a powerful tool in combating cancer. Because of its specificity, it may prove to be more advantageous over the currently used drugs which may have unwanted toxic side effects on the CNS. In the near future, SVCT2 could have the potential to be adopted as a promising therapy against cancer and certain tumors. Furthermore, SVCT2 has the potential to be applied to many situations and can be modified to fit a number of situations that deal with getting past the Blood Brain Barrier. Initially, SVCT2 was only modified with three forms of treatment for Glioblastoma Multiforme, STI-571, CCI-779, and SAHA, however there are countless other treatments that have been developed, but that are not in use due to the BBB. This project was successful in determining an appropriate temperature of 37°C for the procedure to be used. The limitations of this experiment include the fact that this experiment was performed in vitro and so complexity among individuals cannot be analyzed. However, this is an early step for the future of SVCT2 as a treatment, and clinical trials to test SVCT2 in vivo may not be too far off.

此次實驗發現蘋果能夠引發康乃馨產生睡病，以此證實蘋果具有催熟其他植物的能力，並推測是因為蘋果會釋放乙烯之故。此外，比較不同狀態蘋果催熟效果的差異，結果催熟能力：整顆蘋果＞切碎蘋果＞蘋果皮＞削皮蘋果。

氣候學是研究對地表長時期氣象觀測數值所求得平均狀況的科學，在大氣科學的領域中自成一個體系，地中溫度為氣候學範圍內的一個環節，通常在氣候學中很少去討論，本研究試從地中各層面的溫度的逐日變化、季節變化及年變化等不同角度去探討分析，旨在發現地下各層面的特徵。本研究產生的動機是因為常我們觀測地中溫度時，對每個不同深度的溫度計所產生溫度的差別以及不同時問內的溫度升降變化發生興趣，經過初步研究的結果始發覺它們都有規律性與週期性，當我們把這個現象報告老師，老師才鼓勵我們對這個問題做更深一層的探討。本賓驗主要探討的目標是由地面到下層 500 公分深處之問各個層 面，地中溫度升降起伏的變化，並求得短時問的氣溫升降所能達到地下的深度，冬夏季節性的溫度變化對地下溫度所產生的影響。本校的氣候觀測站，因儀器簡陋，所得資料難免有誤差，所以本研究僅強調方法的進行和理論的證實。

我們住的地方附近寺廟共有十五處之多，每天經過那裹總看到廟宇香火繚繞，善男信女又是燒香膜拜，又是口中念念有詞彎腰「卜杯」。記得台灣省主席謝東閔先生曾經要我們破除迷信，雖然我們也看過「迷信害人知多少」這本書，但是，目前多數人還是執迷不悟，每遇到家裹大小事總愛在神的面前「卜杯」禱告以求神的指示，然後按照所謂神的旨意去做，有的人做事順利，他說是神很靈杯很準，做得不順利，還自圓其說的為神辯解說是自己不小心啦！或劫數作祟運氣不佳啦！一點都不敢埋怨神的不是，「到底用杯箋占卜可靠嗎？」大家都有興趣想追求它的究竟，請教老師，老師要我們做調查實驗研究，於是我們幾個人利用春節期間相約到附近寺廟去參觀，想得到一個比較滿意而合乎科學的結論。

洗潔劑分散系泡膜的變化十分有趣，是否能用吹泡法比較表面張力大小？沾黏在立體的框上會怎麼樣？能否由框上薄膜面積大小討論界面劑的化學性質呢？