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深思熟「綠」才會螢-葉綠素螢光的探討
利用簡單的濾紙層析法,可從植物葉片中萃取光合色素的成分,而利用紫外光照射以及紫外-可見光電子吸收光譜和放光光譜的測量,可判斷各成分所得結果是否有所差異。另外,我們試著加入純水或數種不同金屬離子配製的溶液來檢測對葉綠素螢光強度的影響。我們也嘗試著將萃取出來之葉綠素汁液製作成趣味隱形墨水,書寫在紙上的筆跡在一般光線下是無法以肉眼觀察到,特別的是,在暗室中經過紫外線照射後,便可清楚的看到所有紙上的筆跡。
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實驗探討影響共鳴空氣柱有效長度的變因
高中物理實驗十五共嗚空氣柱﹝註1﹞,已知頻率 f 的音叉在圓柱形共鳴管口振動,調整共鳴管水位,當有最大共鳴聲時,量出空氣柱長l=λ/4,由聲速Vt=fλ=4fl和Vt=331+06t﹝t為空氣溫度﹞做比較,結果相去甚遠。另取玻璃瓶(非圓柱狀)盛水,相同音叉在瓶口振動,共鳴時的空氣柱長度和圓柱型不同,引起我們進一步研究的興趣。
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土壤中的水精靈-探討捷運大安森林公園站的土壤液化防治
本研究在於模擬臺北捷運大安森林公園站土壤液化現象並探討動力夯實工法防治效果。本研究分兩部份;一、預備實驗;二、實驗研究。預備實驗為設計沉積土層箱和捷運站砂土箱,並模擬土壤液化現象。實驗研究主要為了探討動力夯實工法對臺北捷運大安森林公園站砂層的效果。由預備實驗,我們設定1500mL水量、砂土深度10cm、砂層中未有緻密岩層,進行臺北捷運大安森林公園站砂層模擬,可以發現施作動力夯實工法砂層可以有效防治土壤液化,並從實驗中我們發覺經地盤改良的砂層在地震後會有地表濕潤現象,因此我們建議施作動力夯實工法後的砂層上方可以鋪設植草磚或連鎖磚,利於行人走動。
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向"毒品"說不-簡易安全家用香辛料殺蟲液
本試驗利用一般家庭常用之香辛料,如:蔥、青蒜、分蔥、芹菜、洋蔥、芫荽、辣椒、羅勒、薑、檸檬、蒜頭 等十一種香辛料植物以一般家用徒手操作之研磨器研磨得原汁,本試驗選取三種不同分類屬性之蟲體結構,進行防除試驗;(1)紋白蝶幼蟲(屬”鱗翅目”昆蟲)、(2)黃斑粗喙椿象(屬”半翅目”昆蟲)及(3)玉米蚜蟲(屬”同翅目”昆蟲)之蟲體上進行驅殺蟲試驗。試驗分三階段進行:第一階段 原汁噴灑試驗:以原汁噴灑蟲體,觀察其對蟲體之影響。發現辣椒、蒜頭、薑香辛液,對此試驗之三種蟲皆有驅殺功用。其於蔥、青蒜、分蔥、芹菜、洋蔥、芫荽、羅勒、檸檬等,對此試驗之三種蟲則無有效之結果;蟲體噴灑香辛液後,呈現嚴重不正常之活動情形,如:蟲體自動呈現腹部反轉朝上,後肢微微抽動扭曲或蟲體靜止活動,並可確定為死亡。由觀察得知,辣椒、蒜頭、薑具直接性觸殺劑(高濃度原汁施用一次,即可殺死蟲體)或殘效性觸殺劑之殺蟲功能(低濃度施用多次後,累積劑量才可致死)。第二階段 稀釋液噴灑試驗:為節省成本之考量,針對第一階段所得知試驗結果,選取辣椒、蒜頭、薑等三種香辛料進行稀釋液殺蟲試驗。結果發現,如:水:辣椒(體積比)1:2、水:蒜頭(體積比)第三階段 田間試驗:為確實印證香辛液於田間使用之可行性,此階段試驗根據前兩階段之試驗結果,施行於田間之結球甘藍葉面之紋白蝶幼蟲蟲體,可獲致有效驅殺蟲體之效果,證明本試驗於實際運用之可行性。本試驗以尋求簡易、方便、安全之家庭園藝用驅殺蟲液為目的,適合推廣應用為一般家庭小面積之園藝栽培,其具操作簡便之特性,並可達確保人體食用及居家安全之效用。
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The Look and Say sequence is produced by describing the appearance of the previous row. For example, start with “1,” which can be described as “one 1,” and therefore the second row is “11,” which is "two 1s," making the third row “21,” the fourth row “1211,”and so on. The main goal of this study is to work out the exact formula for this sequence, which means given the row number n, we can know at once what the n-th row is without having to start from the first row and doing the look-and-say iteration for n-1 times. Some of the methods used include dividing groups, repetition and cracks. The formula we derived speeds up the calculation and gives us a better understanding of the look and say sequence.「外觀數列」為依照外觀產生下一列的數列,第一列為「1」,第二列描述第一列「1 個1」而為「11」,第三列則描述第二列「2 個1」而為「21」,第四列「1211」,依此類推。本研究針對外觀數列的各項數學性質作研究探討,並由此推導出外觀數列的一般式,即給定第n 列就可知道該列的內容。我們運用了分組、重複性以及裂縫的方法分析數列,最後得到了其一般式,此一般式有助於運算速度的加快以及我們對數列性質的了解。
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因應發生天然災害的頻率增加,為解決在沒電、沒瓦斯的情況下,災民還能獲得熱騰騰的飲食,研發救難時用的冷水泡麵是必要的。 而泡麵要能煮熟,必須浸泡在高達80℃以上的熱水中,且持續3分鐘之久。為此,我們進行一連串的實驗,發現生石灰與水需在固定的比例下,才能滿足將泡麵煮熟的條件。同時,我們也探討了水溫、容器材質等變因對反應速度的影響,最終找到了傳遞溫度最快的內容器材質與保溫效果最佳的外容器材質。
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The spiders, Neoscona nautica, often appear in groups, but individuals have their own sense of territory.They usually spin webs among branches during 6:00~ 8:00 in the evening. When building webs, they will first start with bridges and then spin Y-shaped spokes. Next, they spin meshe of net, silk frame, spokes, spirals and free-zone in order. After finishing webs, they will wait for prey on the free-zone or meshe of net. If they find something inanimate on the web, they will break the spiral attached with the inanimate object that is later removed. If the meshe of net is broken, they will fix it immediately. For them, the time to take webs back is during 2:30~ 6:00 in the morning. Most time they use the first pair and the second pair of legs to take webs back and swallow the webs. Sometimes, they break the spirals by the last pair of legs. The sequence to take webs back is : lower right section, lower middle section, lower left section, upper left section, and upper right section. At last, one thread of bridge will be left. Every early mornings they take webs back and swallow them. The next evening they rebuild webs. Possibly there are two reasons to explain why spiders eat their webs: (1).They swallow webs to get protein. (2).The web threads are easily polluted by dust and humidity and reduce stickiness. The web may also reduce the probability of capturing prey. The body length of them is not related to effective web dimensions. However, the web sizes depend on the width of web-building location. The study shows linear relation among body length, meshe of net and dimensions of free-zone. The linear relation represents that the meshe of net and free-zone have ecological or survival meaning for them. We expect that this study of Neoscona nautica can be helpful to build spider ecological database in Taiwan.簷下姬鬼蛛常成群出現,但個體卻有很強的領域性;常於下午6:00 至8:00 結網於樹枝間,結網時,先以橋絲為出發,織出一Y 形的縱絲,再由此依序織出中空網眼、絲框、縱絲、橫絲、棲息圈,網結好後,簷下姬鬼蛛則在棲息圈或網眼靜候獵物,若發現網上有非生物之異物,則將黏住異物的橫絲弄斷,再把網上的異物丟棄;若網眼被破壞,則會立即修補。收網時間為凌晨2:30 至凌晨6:00,收網時,大部分由第一、二對步足進行收網,偶爾會用最後一對步足將橫絲弄斷,一邊收網一邊將網吞食,收網的順序為:右下、中下、左下、左上、右上,最後留下一條橋絲。簷下姬鬼蛛每天清晨都會收網,並將網吃掉,翌日傍晚再重新結網,其可能原因有兩點:(1)將網吃掉以補充蛋白質。(2)蛛絲容易受灰塵、水氣之污染而減小黏性,降低獵捕功效。簷下姬鬼蛛體長與有效網面積無關,但網的大小視其結網地點寬敞程度而定。體長與網眼、棲息圈面積呈線性關係,表示網眼和棲息圈對簷下姬鬼蛛具有生態或生存意義。我們對簷下姬鬼蛛生態調查之結果,希望能幫助台灣的蜘蛛生態資料庫之建立。
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我們發現乾溪不應該潰堤-桃芝颱風造成大屯區河川災害之研究乾溪潰堤處良田淹沒情形
發生在90.7.29-7.30 的桃芝颱風,造成南投、花蓮地區嚴重的土石流災難,同時桃芝颱風也給台中縣大屯區帶來慘重的災害,其中匯流入大里段草湖溪的支流乾溪二、三十年來首次潰堤成災,2 人死亡,200 公頃農田遭土石流淹沒,2000 多戶人家淹水,災情相當嚴重!
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Remote Activated Chip-Based Drug Release System Using Nanoparticles as an Anti-Cancer Therapeutic
The pharmaceutical industry is constantly searching for methods that allow drugs to be delivered as a direct response to a specific stimulus, in which the locus of delivery is in the vicinity of required region. A unique thermo?reversible hydrogel,F127 modified with dimethacrylate (DMA), that can deliver drugs at physiological temperatures was synthesized. Nanoparticles which are specific for targeting human body cancer cell were absorbed by this hydrogel. The toxicity of nanoparticles with different diameters and coating was measured using the MTT assay. It was found that nanoparticles with smaller diameters and folate coating were most toxic to the cancer cells. The release rate of the nanoparticles from the hydrogel was measured as a function of temperature with the hydrogel releasing approximately 3 L nanoparticles per hour. Exposing this drug delivery system to cancer cells would effectively inhibit MCF7 cell proliferation. By grafting this nanoparticle?loaded hydrogel onto a thermoelectric module, the release of the drug would be controlled. Thus, a successful temperature sensitive hydrogel was synthesized that releases cancer?targeting nanoparticles which inhibit cancer cell proliferation, thereby engineering a controlled drug delivery system.
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日照影響花芽分化及生長試植物生理學上的重要課題,根據相關文獻指出,蛋白質類型的變化是影響開化的重要關鍵,大豆是短日照植物,分別以長日照(LD18hr/DAY)和短日照(SD6hr/DAY)處理,抽取葉片中的蛋白質以SDS-PAGE.2D-SDS-PAGE分析,SDS-PAGE的實驗姐果顯示出在分子量25.2 32.9 38.6 20.6 64.0 100.6 kDa處中的蛋白質SD比LD的量多,但是在分子量16.8kDa以下的蛋白質LD含有高濃度而自SD只有一點,我們使用2D-SDS-PAGE做進一步的分析,SD與LD分為三個區塊討論(SD1-3 LD1-3)有許多蛋白自LD1.LD3出現,但在SD1 SD3中未出現,可能有抑制花芽分化的作用,而在SD2中有多個高濃度蛋白出現,可能在花芽分化上扮演重角色,其中編號P1 P2的蛋白質有較明顯的變化,根據NCBI網站上的資料顯示,P1蛋白質與光敏素C有關,而P2蛋白質則沒有找到分子量等電點類似的蛋白質;The effects of photoeriod on floral initation and develoment is an imporant phyiological subject and have studed ve he past 70 years. Accrding to the reference, polypeptide partten plays an important rolein photoperiodic induction. Soybean s a SD(short day)plant. So we planted them with 6-h(short day,SD)and 18-h(long day,LD)photoperiods. Total proteins n leaves of soyban were extracted from the vegetable,SD and LD. And using SDS-PAGE and 2-D eloctrophoresis to analyze. 25.2,32.9,38.6,50.6,64.0,and 100.6kDa polypeptides in SDS-PAGE were always present in leavess at SD but only a little bands f the polypeptides appeared at LD. And many polypeptides whose molecular weight(Mw)is below 16.8 kDa had higher concentration at LD ad Reduced at SD. We found three different areas(SD1~3,LD1~3) and analyze them. Many proteins were found n LD1,LD3 and reduced in SD2,SD5. Possibly, these polypeptides found in LD1 and LD3 play an important role in restraining floral iniiation in soybean. Many proeins had more concentration in SD2. Probably, they play an important role in photoperiodic induction and floral initiation. We found a protein labeled P1 in SD2 and it is with molecular weight(Mw) of 36kDa at isoelectric point(pI) of5.2, and it may be related to cytochromeC. We also found protein labeled P2 in SD2 and it is with molecular weight(Mw) of 40.2kDa at isoelectric point(pI)of 5.4, and we couldn't find any protein that has the similary Mw and pI in potein database about flowering and daylength.
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妙筆生花─防斷水又能矯正握姿的原子筆套
本研究主要目的: 一、了解原子筆斷水及寫字不好看的原因。二、探討及找出原子筆斷水和字寫不好看的辦法。三、尋找自製原子筆最佳材質及測試並調查滿意度。 本研究主要實驗: 一、模擬鋼珠頭書寫。 二、自製原子筆與市售原子筆摔幾次會斷水。三、自製原子筆套是否會彈性疲乏。 四、滿意度調查。本研究主要結果: 一、 原子筆斷水主因為鋼珠撞擊地面而內縮、變形;字醜的主因為握筆姿勢不正確。二、 市售原子筆摔70次時斷水;自製原子筆摔150次仍無斷水,顯示自製原子筆較不易斷水。三、開花設計一小時後保護管無法彈回,顯示自製原子筆有彈性疲乏的問題。四、經比較,筆套材質用較硬吸管較為合適。五、自製原子筆能加強握筆力道,固定、改變握筆姿勢。
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