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熱門關鍵字: the king 水果 豆漿 電腦
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蟋蟀的聲音分析與聲音行為探討

蟋蟀聲音與行為的關係密不可分。取北部四種蟋蟀樣本,以數位錄音筆錄製及電腦軟體分析蟋蟀的聲音,並用PCR技術萃取蟋蟀的粒線體DNA(16S)加以分析,藉此和傳統的形態學分類相互比較。結果發現蟋蟀聲音的頻率特性中主頻率的差異在親緣遠近有關聯性,但聲音的時間特性方面則沒有特定關聯,不過聲學分析圖仍可作為單一種的鑑定指標。此外,研究發現此4種蟋蟀的聲音頻率範圍有重疊現象,但主頻率、脈衝比、唧聲率及唧聲長不同,推測雌蟋蟀應是綜合這些特性辨別同種。另一方面,以黑蟋蟀作為研究對象,進行干擾實驗,觀察並繪製出其聲音行為模式; 並將此作為對照,結果發現蟋蟀的生殖行為主要受到雄蟋蟀的聲音支配,而雄蟋蟀則以嗅覺辨別雌蟋蟀位置,決定下一步行動,與視覺較無關係。 Cricket's voice is closely related to the behavior. We select four species of crickets from the northern Taiwan to study the acoustic and mating behaviors. The digital sound recorder and acoustic software are used to analyze their acoustic characters. Furthermore, the PCR technology is used to amplify and sequence parts of mtDNA sequences and the results were compiled as the comparison to the traditional morphological character. The result shows that the resolution in acoustic characters of main frequency, pulse number, chirp rate, and the length of chirp are different among four species, though the minor frequency pattern is overlapping. Moreover, we find that frequency characteristic difference among four species is partly related to their phylogenetic relationship, yet is not seen in time characteristic. It is obvious that difference of acoustic behavior can be regarded as identified index among species. Results of acoustic analysis impel us to infer that the female crickets take the comprehensive acoustic behavior to distinguish their specific male. In addition, behavior pattern and model of Gryllus bimaculatus are established as the comparison of interfering experiment is undertaken. Which results suggest that the mating behavior in cricket is dominated by males’ acoustic behavior, and is initiated by male’s olfactory sense rather than that of sight.

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被遺忘的黃金-添加豆渣製作土司之探討

以黃豆製作豆漿時,留下的豆渣中各種營養成分(粗蛋白、粗脂肪、灰分、碳水化合物等)之殘留率不低,豆渣有回收再利用之價值。豆渣較適之處理條件:溼豆渣先經粗篩,再以120℃熱風乾燥,豆渣之香氣、色澤、乾燥效果均佳。以豆渣取代15%之麵粉時,消費者喜好程度最高,但豆渣取代麵粉含量越高,則土司之高度卻越低。若改為額外添加15%豆渣之製作方法(麵粉含量不變),明顯改善土司高度過低之問題。測試土司內部組織之質地,發現豆渣土司硬度明顯高於白土司。官能品評試驗結果顯示,不同年齡、性別或整體消費者,對豆渣土司、白土司之喜好程度相近,並無顯著差異。

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Developing Swarm Intelligence with Flying Robots for Life Saving

Purpose of the research I had a bicycle accident eight months ago and I suffered an opened humeral fracture. I wasn’t able to get up on my feet, I had to suffer on the ground while a couple accidentally walked there, and they called the emergency services, I lost a lot of blood and the doctors had to cut a small piece of muscle out of my biceps. Now this shouldn’t have happened if there were a faster method on making first aid. Example an intelligent flying robot could easily handle this situation. Procedures There is a hexcopter and a quadcopter. Both of them has cameras with live image transmissions in Full HD and they also have GPS. They are equipped with a lot of sensors (image recognition with Raspberry Pi, 6 axis distance sensors, long range communication modules) and with these they are able to solve problems just a bird or a human does. These drones can communicate up to 1.8 kilometers, they communicate with the main server, they share their knowledge with each other and this way they can learn about the world and about the terrain that surrounds them. In a very basic way they are able to learn… Each time they make their flying techniques smoother and their database is growing. These drones can cooperate and save lives faster than humans do. They are also able to fly in non-accessible zones by themselves with no human help. For example with their sensors they can fly in and map buildings that are inaccessible for people. They can also do artificial farming with their object recognizing features, like recognizing plants and colors. (Right now they are calibrated to look for survivors alone in the mountains/forests.) They go to a given GPS coordinate then they are flying in a spiral form to find the injured people in less than a half hour. I really hope that one day these flying intelligent machines will save lives! Note: (I probably can only take one drone with myself to Taiwan, because of the traveling restrictions on planes.)

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生物濾水器 ~ 重金屬吸附能力

重金屬會直接或間接影響植物的發芽,將綠豆浸泡在不同濃度與種類的重金屬離子(銅、鋅、鈷、鉛、鎳)溶液下,我們發現浸泡濃度愈高,綠豆的發芽率愈低,而銅離子對於綠豆發芽率的抑制效果最好,鈷離子的抑制效果最差。由實驗結果得知重金屬濃度與綠豆發芽率有明顯的負相關,也就是重金屬濃度愈高,綠豆發芽率愈低,利用推算的回歸曲線,可以由綠豆發芽率來估算重金屬濃度,是一個簡單又經濟的生物檢測方法。文蛤與牡蠣屬於濾食性動物,靠過濾水中懸浮性有機物而生存,水中的懸浮物質會吸附一些污染物,如:重金屬或有毒物質,這些懸浮物質會被濾食性動物體內,文蛤對於的銅、鋅、鈷、鎳離子的吸附能力皆明顯,只有對於鉛離子的吸附能力較差,而牡蠣對於銅、鋅離子的吸附能力較大,對於鉛、鎳離子吸附能力較小,而對於鈷離子幾乎沒有吸附能力。

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線上教學資源

健康促進學校簡介

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光電化學能車~自製電池的探究與應用

高價能源時代來臨。尋求永續、環保、經濟的能源刻不容緩。太陽能乾淨無污染,但遇上陰天或雨天便要罷工。本件作品主要「以水果電池為基礎,尋求安全環保的電解質;普遍、便宜的陰極、陽極材料;以較小的體積自己製作較大電流與較高電壓的環保電池,並加以實際應用於太陽能車上,作為預備電源,使成為全天候不虞電力中斷的光電化學能車」。

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窮人的人蔘?

白蘿蔔含豐富維生素 C(抗壞血酸),但不能與紅蘿蔔一起煮,主要原因是紅蘿蔔含抗壞血酸氧化?。對這個問題做進一步探索。利用滴定方法,可以測定白蘿蔔或紅蘿蔔中維生素 C 的含量。同時也可以測定白蘿蔔的抗壞血酸如何受紅蘿蔔抗壞血酸氧化?的作用。我們研究白蘿蔔及紅蘿蔔燉煮時加醋或分開燉煮,看是否降低了白蘿蔔維生素 C 被紅蘿蔔抗壞血酸氧化?破壞的程度!在市面上的泡菜,可以看到紅蘿蔔及白蘿蔔一起醃製,他們會互相作用嗎?蘿蔔乾在客家人的眼中被視為「窮人的人蔘」。我們真想知道維生素 C 的變化!測定不同年份的蘿蔔乾,我們發現越陳年的蘿蔔乾,維生素 C 的含量越高!既然維生素 C 加熱會被破壞,而放越久,維生素 C 反而增高呢?

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線上教學資源

運用Arduino探究視覺暫留

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磁性流體可調性折射率特性之研究與應用

磁性流體(magnetic fluids)是一種含有磁性奈米粒子的液體,當磁場外加於磁性流體時,流體中各磁性奈米粒子的磁矩會沿外加磁場方向排列,而導致粒子間相互吸引,形成較大的磁性叢集,即所謂的磁鍊。當外加磁場增強,該磁鍊數會變多,並使磁性流體的折射率產生變化。磁性流體的折射率變化會隨外加磁場之變大而增大。本研究除探討磁性流體折射率受外加磁場控制的變化情形及其物理原由外,並進一步運用此特性研發可調性光纖「光調制器」 ,以探討磁性流體可調性折射率應用在光電元件上的可行性。A magnetic fluid is one kind of colloids which contain magnetic nano-particles. Under an external magnetic field, the magnetic moment of nano-particles is aligned along the direction of the external magnetic field. This leads to the agglomeration of magnetic particles and to form magnetic clusters under an external magnetic field. With the formation of the magnetic clusters, the refractive index of magnetic fluid is varied. The refractive index of magnetic fluid was found to increase under a higher magnetic field. In this work, In addition to investigating in detail the behavior of the field-dependent refractive index of the magnetic fluid, we also explore the relevant physical origins. Furthermore, the feasibility of developing fiber-optical modulators by utilizing the tunable refractive index of magnetic fluids is discussed.

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Discovery of new bioactivities of violacein and its erivatives synthesized by Chromobacterium sp.

目的:紫色桿菌(Chromobacterium sp.)是一種生活在土壤及水域環境的革蘭氏陰性菌,它會產生紫黑色的紫色桿菌素(Violacein),因而使菌體與培養液皆呈紫色。Violacein是一種由兩個L-色胺酸(L-tryptophan)分子所聚合成的五環化合物,已被證實具有抗菌以及抑制腫瘤細胞生長的活性。本實驗的目的在探討紫色桿菌是否會利用不同官能基的L-色胺酸,合成不同的Violacein衍生物,並分析這些Violacein衍生物的生物活性。方法:將紫色桿菌培養於液態LB培養基中,分別加入不同濃度的L-tryptophan、1-CH3-L-tryptophan與5-OH-L-tryptophan,於室溫下培養48小時,再用乙酸乙酯萃取Violacein及其衍生物,將萃取出來的紫色桿菌素,用紫外光/可見光分光光譜計和質譜儀進行分析。最後,再將這些萃取物打入斑馬魚受精卵做胚胎毒性測試,並進行試管外DNA剪切能力分析。結果:加入不同官能基的L-色胺酸所合成的產物都是紫色,且在紫外光/可見光的光譜中的最大吸收波長皆相同,質譜儀分析結果則顯示,L-tryptophan 、與5-OH-L-tryptophan的產物圖譜不同、而1-CH3-L-tryptophan的產物則無法分析。斑馬魚胚胎毒性測試結果顯示,Violaecein和1-CH3-Violacein 不會對胚胎發育造成影響,但是5-OH-Violacein則會造成胚胎發育異常。DNA 剪切能力測試也顯示,只有5-OH-Violacein具有剪切DNA的能力,其他則否。重要性:本實驗成功地利用紫色桿菌合成Violacein及其衍生物,並發現了5-OH-Violacein比Violacein 具有更高的生物活性。;Aims: Chromobacterium sp. is a Gram negative bacterium which inhabit in soil and water environments. Chromobacterium produce a purple color compound named Violacein. This colorful compound makes Chromobacterium and its culture mediumin purple color. Violacein is a five rings compound synthesized from two L-tryptophan molecules. Studies have shown that Violacein has anti-bacteria and anti-tumor activities. The purpose of this study is to investigate whether Violacein can be synthesized from different tryptophan analogues, and whether these Violacein derivatives have different bioactivities. Materials and Methods: Chromobacterium sp. was cultured in liquid medium containing different concentrations of L-tryptophan, 1-CH3-L-tryptophan, and 5-OH-L-tryptophan. After 48 hours incubation at room temperature, Violacein and its derivatives were extracted by EA, analyzed by UV/visible spectrophotometer and MS. Violacein and its derivatives were also tested for their embryo toxicity and DNA cleavage activity in vitro. Results: The compounds synthesized from different tryptophan analogues were all purple and have the same maxima absorption wave length in the UV/visible spectra. However, MS spectra of compounds synthesized from L-tryptophan and 5-OH-L-tryptophan were different. The results of zebrafish embryo toxicity tests indicated that violaecin and 1-CH3-Violacein had no effect on embryo development, but 5-OH-Violacein caused development defects. DNA cleavage tests also showed that only 5-OH-Violacein could digest DNA in vitro. Violaecin and 1-CH3-Violacein could not digest DNA. Significances: Results from this study indicate that Chromobacterium sp. can be used to synthesize Violacein derivatives from different tryptophan analogues, and revealed that 5-OH-Violacein is a higher bioactivity compound than violaecein.

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魔術「方塊」變「平面」

我們由數學課的拼組四格平面魔術方塊,發現平面魔術方塊的組合問題。我們想瞭解並解決平面魔術方塊的變化問題,同時發展有趣的不同平面魔術方塊。結果我們發現:1.平面魔術方塊四格位置無法全部排列出來。2.割線會影響平面魔術方塊的拼組。3.平面魔術方塊數字的位置是有規則的,只要是規則中的方法,均可以折的出來。4. 我們發展出(1)二維平面魔術方塊-十字型(+)、田字型(■ )、田字中空型(□)、E 型(E)、L 型(?)、H 型(H)、T 型(T)。(2)三維連續型魔術方塊。我們認為平面魔術方塊的對折翻轉變形特性極富學習意義,激發我們的想像力以及創造力。

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燃料電池用之磺酸化SEBS-奈米粒子複合膜製備與性質研究

聚苯乙烯(S)-聚乙烯(E)-丁二烯-1(B)-聚苯乙烯(S) (polystyrene-block-poly(ethylene-ran-butylene)-block-polystyrene,簡稱SEBS)是目前直接甲醇燃料電池的質子交換膜研究中常用於與Nafion比較的膜材。Nafion的價格昂貴,且其高甲醇滲透率將造成電極毒化,故本研究探討不同製程與添加不同奈米粒子所製作之質子交換膜特性,以有效降低甲醇滲透。結果發現,添加之ZrP為層狀結構,增加甲醇通過親水區域端的難度,能有效阻擋甲醇滲透。且藉由磺酸根官能基團,以及本身帶有質子傳導性質,所以與Nafion的PTC數據差距不大,因此C/P值較Nafion高。未來可改變不同磺酸化程度,並在SSEBS內添加不同奈米粒子,並對於質子交換膜的持久性及實際應用之探討。

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