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Blood Brain Barrier Breached!
The purpose of this project is to determine if it is possible to use Ascorbic Acid Sodium- Dependent Vitamin C Transporter Type II, SVCT2, as an effective and safe protein to attach to certain brain tumor treatments to bypass the Blood Brain Barrier (BBB). Stemming from this problem, a procedure was created to use in vitro engineering with the aid of a professor at the University of Calgary to combine SVCT2 and three specific tumor treatments; Imatinib Mesylate (STI-571), Temsirolimus (CCI-779), and Suberoylanilide Hydroxamic Acid (SAHA). Following this, a metabolic barrier had to be created to simulate the BBB. To do this, the use of three enzymes were mixed and held together using specific bonds. Finally, a special bio-tracer was placed within the barrier to detect any toxic effects that may be produced. Then two trials were made with each treatment on the barrier at 34°C, 37°C, and 39°C. Once this was done observations could be made. When the newly isolated SVCT2 attaches to the three cancer treatments, they would all be able to connect and form bonds with each other. Once the incubation period is over for the first trial at 34°C, 37°C and 39°C, several things would be observed within the data. When counting the number of cells that were able to get into the engineered metabolic barrier, it could be seen that there was a dramatic increase in the number of cells in the 37°C range. SVCT2 can be a powerful tool in combating cancer. Because of its specificity, it may prove to be more advantageous over the currently used drugs which may have unwanted toxic side effects on the CNS. In the near future, SVCT2 could have the potential to be adopted as a promising therapy against cancer and certain tumors. Furthermore, SVCT2 has the potential to be applied to many situations and can be modified to fit a number of situations that deal with getting past the Blood Brain Barrier. Initially, SVCT2 was only modified with three forms of treatment for Glioblastoma Multiforme, STI-571, CCI-779, and SAHA, however there are countless other treatments that have been developed, but that are not in use due to the BBB. This project was successful in determining an appropriate temperature of 37°C for the procedure to be used. The limitations of this experiment include the fact that this experiment was performed in vitro and so complexity among individuals cannot be analyzed. However, this is an early step for the future of SVCT2 as a treatment, and clinical trials to test SVCT2 in vivo may not be too far off.
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Titania Nanotubes for Solar Energy and Catalysis
Introduction The discovery of titania (TiO2) nanotubes suggests vast improvements over extant titania properties. Titania nanotubes are aligned in highly-ordered arrays with a large geometric surface area, making them the ideal material for many applications. However, the mechanism responsible for the growth rates of highly-ordered nanotubes with optimal adhesive properties is not fully explained or understood. Purpose of Research The aims of this project were threefold: to explore the effects of different anodizing parameters on the fabrication of titania nanotubes; to study the photocatalytic activity of the nanotubes; and to deposit gold nanoparticles into the nanotubes. Methodology Nanotube Fabrication: Titanium foil was subjected to potentiostatic anodization with the use of various fluorinebased electrolytes, anodization voltage and duration to compare the effects of different parameters. Scanning electron microscopy (SEM) was used to characterize the nanotube diameter and length of the anodized samples. Photo-electrochemica1 Water-splitting: A PEC cell was assembled using the nanotubes as the photoanode and the samples were anodically polarized in a 1M KOH electrolyte. A potentiostat was employed to control the applied bias and to measure the photocurrent response under light irradiation. Overall photoconversion efficiency (ηc) of the samples was then calculated. Catalyst Support: A gold precursor solution was prepared with HAuC14‧3H2O. Using a novel depositionprecipitation (DP) protocol, gold nanoparticles were deposited on the nanotubes. SEM was used to scan for traces of gold and their locations. Energy-Dispersive X-ray (EDX) spectroscopy was used to confirm the identity of the gold nanoparticles. Data and Discussion Nanotube Fabrication: Preliminary studies found the glycerol/water and glycerol/formamide combinations to be the most promising. In glycerol/water-based electrolytes, higher water content corresponded to a decrease in nanotube length while higher anodization voltage resulted in a significant increase in tube diameter and length. In glycerol/formamide-based electrolytes, higher water content corresponded to a decrease in nanotube diameter while higher fluorine concentration resulted in an increase in inter-tubular spacing. The effects of various fabrication parameters were better understood, contributing to greater control over array dimensions. Photo-electrochemical Water-splitting: A higher anodizaion voltage resulted in a significant improvement in photoconversion efficiency. However, this trend was reversed in chlorine-doped samples, where a longer anodization duration corresponded with better photoconversion efficiency. Doping was found to enhance the photoresponse of the samples, with 6.32 % photoconversion efficiency obtained, suggesting new strategies for light harvesting and a step closer towards commercially-viable solar energy. Catalyst Support: Gold nanoparticles (5-10 nm) were successfully deposited onto the titania nanotube samples. Based on current literature, this was the first successful attempt at depositing gold nanoparticles into titania nanotubes. An EDX spectrum confirmed the identity of the gold nanoparticles. Compared to current catalytic converters, the gold/titania nanotube structure offered a larger catalytic surface area for reactants and the ability to function at low temperatures. Conclusion: By understanding the effects of various parameters on titania nanotube fabrication, the anodization process can be optimized to enable more precise control over array dimensions. High photocatalytic efficiency has also been achieved. In addition, doping is found to improve the photoresponse of titania nanotubes. Gold nanoparticles have been deposited, to our knowledge for the first time, onto the surface and inner walls of titania nanotubes.
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本校進行兩棲類的研究觀察已經邁入第三年了,每一種蛙類都深具特色,都令人著迷,但因為我們位處南部,所以對於莫氏樹蛙非常的熟悉,幾乎每隔幾個星期就要去探訪一下每一個地點的莫氏樹蛙。因此這一次科學展覽的主題,就挑定以莫氏樹蛙為主角,進行一系列有系統性的研究觀察,希望能夠揭開山林霧中神秘叫聲主人的生態習性,更希望這些資料與相關研究能夠幫助我們成功延續這已經被農委會列入特稀有保育類生物的生命活力,並吸引更多的人投入自然生態的觀察研究中。
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傳聲筒(一般俗稱大聲公)是在生活中常常可以見到的物品,它能使發出的聲音更加宏亮。本研究的目的即想探索、改良大聲公的各種可能性?所以改變了大聲公的長度、口徑大小、材質、形狀及測試不同位置大聲公的聲音效果,也就是想要進行一項大聲公的終極挑戰,現在就請您仔細看看我們的研究喔!
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線上教學資源
看看「SHERO.我的故事」主題書展
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台灣地區 5 、 6 月梅雨季, 7 、8月颱風來襲,雨量在那段期間比較多,但每年有相當大的差異,到底受什麼因素控制呢?\r 近年來,世界各地頻頻傳出發生不同於往年的氣候現象,例如,秘魯沙漠竟因連月豪雨出現了一個大湖,加拿大發生冰風暴,印尼的霾害,媒禮、科學界的熱烈討論研究,大家都說是“聖嬰現象”惹得禍,使我們一夥人對這個問題很感興趣,於是我們和老師們一同參加一場知名教授的演講,會後又上網查了許多資料。使我們認識了一些氣候上的專有名詞,也知道“聖嬰現象”和“反聖嬰現象”,但是大多為國外的研究,究竟對台灣的氣候有什麼影響呢?我們想了解對降水量、降雨日數以及會造成災害的大雨害豪雨的發生次數有影響嗎?
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本校位於基隆河畔,吾等日常觀察其濁度,似乎一年比一年嚴重,遂發生探其究竟之興趣。
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鏡子是日常生活少不的東西。「以銅為鏡,可以正衣冠」唐太宗的名言流傳千年,鏡子的功能沒有收變,鏡子的製法卻一再更新”現代的鏡子當然不可能是用整塊青銅去磨成。那慶鏡子內層的金屬是什麼呢?如何附上去的呢?某年某月某一天,老師偶然提到鏡子內層的金屬是銀(Ag ) ,一時同學大譁,老師是不是講錯了?臘該是水銀(Hg ) 吧?後來在報上看到一則報導,一位當選傑出十大企業家的鏡廠董事長黃福來先生,年輕時,因製造鏡子,不幸引起爆炸,以致兩眼失明至今。報上也捉到是水銀,到底是水銀或銀?我們還是大惑不解。寒假期間前往鹿港福華鏡廠,向黃董事長加以詢問,有了些概念,但是細節一一例如濃度、用量、溫度、程序 · · · 一因屬商業機密,手邊的一切資料也都一筆帶過,所以只好親自動手去探討。
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假如我是正常的?!—再探渦流脫離是否可能為聖嬰發生動力
聖嬰現象為全球的共同話題之一,由於其發生會影響全球性氣候的改變,使原本乾旱處下起傾盆大雨,原本潮濕氣候的地區成了乾旱地區,導致生命、財產嚴重性損失,這也是引發本研究想站在不同科學領域角度來探就聖嬰現象的發生的原因。目前科學家大都以大氣觀點來說明聖嬰的成因:太平洋上因風向改變引起海水流向或動能變化,導致太平洋赤道地區及南美洲西側海面溫度的改變,而觸發聖嬰的發生。由於海水的熱容量比空氣大,因此我們想以海洋觀點來說明另一種可能觸動聖嬰發生的動力,在生活經驗裡,我們發現水流通過障礙物會在後方形成渦流,因此,當南極繞極環流通過德瑞克通道受到南美洲南端阻礙時,有可能在南美洲形成週期性渦流生成及脫離的現象。「這樣的渦流是否存在?」、「渦流脫離後在南美洲左右岸海面溫度、高度是否發生變化?」、「此動力是否進而觸發聖嬰現象?」,是本研究期待以新思惟解釋引發這個全球現象的另一可能動力。
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SEAWEED ON THE NAMIBIA COAST EXTRACTION OF AGAR & USE OF KELP AS FERTILIZER
In Swakopmund we have a problem with seaweed deposits on our beaches. Tons of seaweed are throwni away yearly. The reason I did this project was to see if the seaweed on the Namibian coast can he used instead of just being thrown away.Seaweed is a rich source of natural minerals and vitamins such as calcium, potassium iodine and also Vitamin A, BI and K. The seaweed on our coastline cannot he eaten as the salt content is too high. Seaweed is a marine algae that works like a sponge absorbing nutrients and minerals from the water.I extracted agar (a jelly) from red seaweed called(GracilariaVerrucosa). I dried the agar and seaweed, and I also grew mushroom spawns and bacteria on the agar plates. Secondly, I researched the possibility of using kelp (Laminaria Pallida), as fertilizer and livestock supplements. The unemployment rate in Namibia is very high. With adequate financial resources and proper marketing, seaweed can he used to increase the economy. We can market the agar to pharmaceutical and cosmetic companies, and also supply it to the university for students in Biology to grow bacteria and other micro organisms. The ferti lizer can be used to boost agriculture in Namibia. It is cheap and the farmers will he able to p1 ant vegetables with better nutritional value.All this was done to promote of biggest natural resource to create more work and to find a way for the less fortunate to create a better life for themselves.
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我們經過二個月的研究,獲得下列重要成果: 一、研發雨刷控制器,傳統雨刷需由駕駛人來操控,我們整合電子-機械控制的功能,當行車遇到下雨天時,控制器能提供電源操作雨刷的擺動,保持駕駛的最佳視線。 二、研發雨刷雨量感知器,如何偵測到雨量的大小及可擺設於汽車前擋風玻璃最佳位置,提升靈敏度,以增加實用性。
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於實際生活情境中發現廚餘液肥會影響蚜蟲的存活,首先對蚜蟲種類及生活習性做探究,接著試驗不同的廚餘化肥菌配方所製造之廚餘液肥對棉蚜的防治效果,發現皆能有效的防治棉蚜。探究蚜蟲可能的致死原因時發現,廚餘液肥對棉蚜的直接致死;而廚餘液肥的氣味對棉蚜的存活並沒有影響;廚餘液肥的酸性物質雖然可能造成蚜蟲的傷害,但應不是主因,推測死因為液肥中含有分解蚜蟲體壁的酵素或可利用菌絲穿透體壁的真菌,另外,菌種配方中所含有的菌株可能本身即可對蚜蟲造成傷害。在實際應用方面,噴灑稀釋100倍的廚餘液肥是屬於經濟又有效的最適濃度,而且只要在棉蚜生活世代內施用一次即可有效的防治棉蚜。以上結果是防治作物害蟲重大的新發現,值得進一步更深入探究廚餘液肥在防治作物害蟲上所扮演的角色。
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