One afternoon, a photography enthusiast, Mr. Cheung was holding some photos, completely absorbed in the fond (but ancient) memories of his honeymoon in Cairo. The badly-yellowed photo prints reminded him of every sweet and romantic moment of the “good old days”. He was soon rudely awakened when Mrs. Cheung shouted, “Honey dear! I was told that high-tech is ‘in’, and the single-lens reflex camera is totally out! So stop hugging the old out-dated pictures.” Mr. Cheung fired back, “Oh, you know nothing. This is something you cannot replace at all, and my camera is a classic.” Smiling at him, Mrs. Cheung took out a new gadget, “Right, right, now calm down. It never hurts to have both worlds. Look what I’ve got you. This is called ‘Stereoscopic Camera Lens’. Its little coloured spectacles can capture everything, say, the sensational Great Pyramid, in three dimensions!” As we are asking for and relying more on technological advances, the Stereoscopic Camera lens and the Stereoscopic Magic Box are surely innovations that promise to add a new dimension to our lives. Surprisingly, the working of these two gadgets involve only some simple scientific theories such as polarization, doping and anaglyph; and employs the special property of Liquid Crystal Layer (LCD) under electric current. Stereoscopic photos can now be easily available to us. Both of our designs, the Stereoscopic Camera Lens and the Stereoscopic Magic Box, can turn something ordinary into extraordinary by making plain, 2-dimensional things ‘stand out’ before your eyes. The Lens itself is crafted with such precision that it can capture any image both faithfully and flawlessly. The Magic Box uses electric current to catalyze and enhance the 3-dimensional transformation. So each picture is vibrant and true-to-life. The Lens and the Magic Box will certainly revolutionize our vision of things around us. To enable you to have a good understanding of the theories behind the making of the Stereoscopic Camera Lens and the Stereoscopic Magic Box, we have made simple models of them with prisms, and they are in display at our booth. Anyone who is interested in these fancy gadgets should definitely come to our booth to experience a new view of the world!

These days we hardly see fireflies, the Milky way, and rainbow. What’s the reason of that?\r We think the reason is related to air pollution but the concrete reason is unknown to us. Thus the purpose of this research is to see the relation between air pollution and rainbow with Aerosol. In this research we approached in some ways about why rainbow didn't appear in polluted atmosphere.

目前市面上缺乏簡單而精準的過氧化氫檢測法，我們參考Fenton Reaction 中，鐵離子對過氧化氫分解的催化模式，利用鐵、鈷、鎳、錳、鋅分子篩，測試他們催化過氧化氫分解反應的效率，從成本、毒性、活化能的多方考量下，選擇以分子篩來固定金屬催化離子，作為我們後續實驗的研究主軸。實驗的初步，我們選擇過氧化氫作為自由基，並著重於過氧化氫的分解反應。利用濃差電池的原理，設計出一套濃度檢測系統，由分子篩作為電極。鑒於粉末狀的分子篩容易流失，我們製備出陶土鐵分子篩，以陶土固定分子篩，並以此作為電極，搭配白金絲，透過能士特方程式，測出過氧化氫的濃度，且藉由電路調控放大倍率，可以直接控制檢測範圍。從實驗結果得知，鐵分子篩在處理過氧化氫的時候，不會有鐵離子溶出的現象，且其催化性在酸性液中可以維持，能不斷的使用，長時間來看，分子篩相當有經濟與環境保護上的價值。We attempted to provide a system for quickly determining the concentration of free radicals. The existing methods or techniques are inefficient or need expensive equipment, therefore, an inexpensive system is being sought for. As a preliminary study, we focused on the decomposition of hydrogen peroxide. Taking the Fenton reaction as reference, we designed a measuring system. This system includes a catalyst containing Fe or Pt ions for catalyzing hydrogen peroxide decomposition reaction. The Fe- and Pt-zeolite were prepared to hold Fe and Pt ions to avoid losing. Because the electrically induced potential would decrease with the decrease in the concentration of hydrogen peroxide, we could measure the concentration of hydrogen peroxide by monitoring the electrical potential. We determined the initial concentration of hydrogen peroxide in water from the initial electrical potential measured through the equation obtained from the calibration line. The practicability of this system has been assured after a series of experiments. We will further develop the technique for measuring other free radicals. We anticipate that this technique will be further developed for measuring other free radicals. Although there are several problems and limitations to be solved and conquered, one thing is for sure: this system is an environment-friendly and cost-effective facility for determining the concentration of free radicals in an aqueous solution.

寬腹螳螂、薄翅螳螂及大螳螂棲息環境多為草叢或灌木，常將卵囊產在植物的莖上。前\r 足、翅膀、頭部、胸部與卵囊等型態上的差異，可用來分辨螳螂的種類及性別。螳螂具有雙\r 眼視覺的複眼會隨著光線改變顏色。螳螂大約經過八、九次的蛻皮後，可變有翅成蟲。母蟲\r 齡數比公蟲多一齡，共九齡。齡數的差別可能為了防止近親交配而產生隱性疾病。螳螂多以\r 若蟲及卵的型態越冬。冬末春初及夏末秋初為草原上大螳螂及薄翅螳螂卵囊的孵化高峰期。\r 螳螂一年四季皆可能進行交配，其中夏秋為交配高峰期。交配時間長達兩小時以上或更久。\r 母螳螂一生中可產2－4次卵囊。由生存曲線得知螳螂屬於幼年高死亡型。螳螂常會清潔自己\r 的頭、觸角、步足等部位。螳螂成蟲喜愛的食物有蝶類、蟋蟀等。受威脅時，螳螂會舉起前\r 足靠在胸部兩側，表現出威嚇狀態。本能趨性方面，螳螂具有負趨地性、正趨光性（黃色光\r 最為明顯）。不同的外界光線顏色和環境顏色，對寬腹螳螂體色改變有不同的影響。

長期以來，臨床研究發現酒精會影響人類胚胎的正常發育，但是其分子機 制尚不清楚。在本研究中利用RNA 定位雜交的方式觀察酒精對於胚胎發育過程 中shh、sox9a、sox9b、col2a1、hand2 的影響，發現這些基因的表現均會受到酒 精的抑制。這項結果顯示在胚胎發育過程中，酒精透過對上述基因的影響，造成 神經脊細胞減少，細胞遷移異常，以及干擾軟骨細胞分化的現象，進而造成頭骨 發育的嚴重缺陷。此外，實驗中亦發現生長激素在腦下垂體的表現亦受到酒精抑 制。這項研究的結果成功地從基因的層次深入了解胎兒酒精中毒症候群造成頭骨 畸形及生長遲緩的病理機制。 It was known that prenatal alcohol exposure may cause serious birth defects and developmental disabilities. The molecular mechanism of this fetal alcohol syndrome still remains unclear. As revealed by whole mount RNA in situ hybridization, it was shown that expression of a number of craniofacial cartilage-related genes, including shh, sox9a, sox9b, col2a1 and hand2, were all inhibited in zebrafish embryo by alcohol exposure. It suggests that alcohol exposure may result in reducing neural crest cell production, interfering neural crest migration, preventing chondrogenesis and eventually cause craniofacial defects. In addition, the transcriptional profile of pituitary hormones were investigated by RNA in situ hybridization. It appears that only growth hormone, but not prolactin and thyroid stimulating hormone, was inhibited by alcohol exposure. The reduction of growth hormone transcription was also confirmed by real time PCR. It also appears that the expression of upstream transcription factor pit1 and downstream target gene igf1 remains unchanged. It suggests that the reduction of gh transcription is mediated by a PIT1-independent pathway. The transcriptional profile of alcohol-exposed embryo was investigated by gene microarray analysis. It appears that the expression profiles of a number of development, cellular signaling, cell growth and apoptosis related genes have be affected by 1.5% alcohol treatment. It was noted that a number of retinal-specific genes were all repressed significantly. It consists with histochemical observation that alcohol exposure results in loss lamination and disturbed differentiation. This study help us understanding the molecular mechanism of fetal alcohol syndrome.

動脈硬化是個致病率和致死率相當高的慢性發炎疾病，為台灣十大死因之一。在病理過程中血中濃度過高的膽固醇為動脈硬化的一大危險因子，其會誘發一連串的發炎反應驅使血管壁內皮細胞功能喪失，血壓上升，平滑肌細胞增生等。Statin 是臨床上十分有效的降血脂藥物，雖然其作用機制已知在於抑制膽固醇合成酵素 HMG-CoA reductase 而有降血脂功效，但近年來探討 statin 在抗發炎方面的作用也漸受重視。nitric oxide synthase (NOS) 代謝產物如 nitric oxide (NO)，cyclooxygenase (COX) 代謝產物如 prostaglandin (PGE?、PGI?)，及 heme oxygenase-1 (HO-1) 代謝產物如 carbon monoxide (CO)，均有文獻指出可以改善血流，而可能在動脈硬化上扮演保護角色。相反的，matrix metalloproteinase (MMP) 的表現會誘使更多的免疫細胞浸潤到血管壁，並增加動脈硬化斑破裂，引起栓塞和中風的發生。在此實驗中，我們利用培養的大鼠主動脈血管平滑肌細胞作為研究材料，發現了 statin (lovastatin 、pravastatin、atorvastatin 、fluvastatin) 具有一些和降血脂無關的直接保護血管壁能力。包括會增加 interleukin-1β (IL-1β) 所誘導 iNOS 蛋白的表現及NO 的產生; statin 本身會增加 COX-2 和 HO-1 蛋白的表現及 PGE? 和PGI? 的產生，及抑制 MMP-2 和 MMP-9 蛋白活性的表現。此外分析調控 iNOS 基因轉錄最為關鍵的基因轉錄因子 NF-κB，發現適量的 statin 會增加 IL-1β 活化NF-κB 的作用。值得一提的是雖然適量 NO 有維持血管恆定的功能，過量時則會造成血壓過低休克的現象，這就是細菌感染後因內毒素 lipopolysaccharide (LPS) 作用引發敗血性休克的主要原因之一。為更進一步釐清 statin 是否會影響受細菌感染病人的生命危險，我們也探討 statin 對LPS 作用的影響。結果發現 statin 反而會抑制 LPS 誘導大量 iNOS 蛋白的表現，NO 的產生及 NF-κB 的活化。這些新的實驗結果提供更多證據支持 statin 可以藉由維持血管舒張，減緩血管壁的發炎反應，穩定動脈硬化斑的作用，以有效控制動脈硬化各個病程的進展。這發現能讓我們更透徹明白 statin 的作用，且對將來研發 statin 在心血管疾病方面新的臨床治療用途是有所助益的。 ;The 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors, statins, are potent inhibitors of cholesterol synthesis and have wide therapeutic use in cardiovascular diseases. Recent evidence, however, suggests that the beneficial effects of statins may extend beyond their action on serum cholesterol levels. In this study, we investigated the effects of lovastatin, pravastatin, atorvastatin, and fluvastatin on cultured rat vascular smooth muscle cells. We found statins can inhibit LPS-induced iNOS expression and NO production, while they can potentiate IL-1β-elicited responses. Moreover, statins themselves can stimulate COX-2 expression, PGE?, PGI? formation, and HO-1 induction. In contrast, statins can inhibit basal and IL-1β-induced enzyme activities of MMP-9 and MMP-2. In studying the activity of NF-κB, which plays an important role for iNOS gene induction, we found that statin can increase IL-1β-induced NF-κB activity, while inhibit that induced by LPS. All these results suggest that stimulation of iNOS expression in the presence of IL-1β, togeth1er with the increased COX-2 and HO-1 expression might contribute to the beneficial effects of statins in atherosclerotic process in terms of vasodilation and inhibition of smooth muscle cell proliferation. The inhibition of MMP activity might enhance plaque stability and reduce the development of atherosclerosis. All these results strengthen the pleiotropic actions of statins in anti-inflammation and anti-atherosclerosis.

本實驗的目的是探討鋼球在磁鐵作用下的效應。本實驗利用「測量臺」與「射出端初速測量裝置」兩個實驗裝置，測量出磁力與距離關係、入射鋼球能量與射出鋼球能量之間的關係。我們以不同的顆數測出射出鋼球所擁有之能量，用不同的固定磁鐵方式比較其能量轉換差異。實驗結果顯示，在經過多組磁鐵加速裝置的實驗後，我們發現射出的鋼球每經過一組加速器，其能量便會等比例增加，這也說明了射出鋼球的能量是具有累加性的。

In order to facilitate a paradigm shift from traditional high volume chemical experiments to an environmentally friendly microdose experiment; I had to innovate and overcome a lot of difficulties. After six generations of experiment design I successfully reduced the volume of the combined reactants to a single drop. I utilized many recycled components to build my apparatus including a vintage computer, a simple CD disk, optical sensors and a transistor from a common computer mouse. By using a CCD monitor with an external camera feed, the single drop chemical reaction can be observed in real time and a recording of the event can be made. I chose the focus of the experiment to be the reaction of sodium thiosulfate and hydrochloric acid to create a colloidal solution of sulfonium nanoparticles. By employing a custom-made transparency target to achieve a higher precision of measurement I have also conducted deeper research into the reaction order and the rate constants. 為了將傳統高劑量的化學實驗順利的推向顯微液滴的化學實驗上，今年我們更是創新突破了很多困難的關卡。 儀器設計由第一代減量研究到第六代的減量設計，目前已能成功的運用報廢的光碟片、報廢的電腦、報廢滑鼠內的感光二極體元件及電晶體來自製設計出兩反應物各一滴溶液做自動偵測反應的記錄。透過顯微鏡加裝的CCD電子螢幕目視觀看、拍成電子影片檔由電腦播放的目標。 因此，我選定了硫代硫酸鈉溶液和鹽酸溶液反應可產生硫奈米微粒的膠態溶液作為實驗的主軸及設計可較精確定量的投影片載液面，我們也企圖對其反應速率式的級數及反應速率常數做更深入的探討。

Arabidopsis thaliana bZIPs（AtbZIPs）是一群影響層面相當廣泛的轉錄因子，一半以上的 AtbZIPs 基因表現受到光的調控，且近九成的分子機制尚未明瞭，因此探討 AtbZIPs 在植物光調控機制中所扮演的角色將是個有趣的課題。AtbZIP16 與 AtbZIP17 皆被推測會參與光的調控機制，然而迄今少有文獻針對這二個轉錄因子進行更多的研究。因此，我們想藉由細菌單雜合系統（Bacterial one hybrid system）的方法，找出能與 AtbZIP16 與 AtbZIP17轉錄因子結合的 DNA 序列，以瞭解此二轉錄因子調節下游基因表現的分子機制，並探討其在光訊息傳導途徑中所扮演的角色。針對 AtbZIP16 與 AtbZIP17，本實驗分別找到了 7 與10 種可能的結合序列。首先，經由資料庫比對分析，我們發現其序列上帶有的 motifs 功能，主要參與在光調控、環境逆境反應機制、組織發育、賀爾蒙調節、病原菌防禦、鈣離子訊息傳遞等方面，其中又以光調控佔最大的比例。再者，藉由將 motifs 的功能繪製成文氏圖，並與 HY5 （AtbZIP56）做比較，結果顯示，這三個轉錄因子雖同屬於 AtbZIP family，據推測皆受到光的調控，可能參與某些相似的生理調節過程，但都各自具備不同的功能，影響植物體的發育。如此的差異，表示他們有實質上的不同，值得我們更深入的研究。整體而言，本實驗結果除了說明 AtbZIPs 的功能確實廣泛之外，也顯示AtbZIP16 與AtbZIP17 是執行光訊號傳導很重要的調控因子。Arabidopsis thaliana bZIPs (AtbZIPs) is a group of transcription factors affecting a wide range of responses in Arabidopsis. The expression of more than half of the AtbZIPs is regulated by light, and the molecular mechanism for roughly 90% of these AtbZIPs remains unknown. Therefore, the roles AtbZIPs play in Arabidopsis light signal transduction is an interesting topic to pursue. AtbZIP16 and AtbZIP17 have been suggested to participate in the regulation mechanism mediated by light. However, only limited studies for these two transcription factors have been previously performed. For this reason, we intended to determine the DNA-binding sequences for AtbZIP16 and AtbZIP17 via the bacterial one hybrid system to reveal their target binding sites in the promoter region of their downstream genes and to speculate their possible biological function especially in light signal transduction pathway. We have identified 7 and 10 possible recognition sequences for AtbZIP16 and AtbZIP17, respectively. Using motif-finding programs analyses, we found the motifs identified are mainly involved in light and stress signaling, tissue development, hormone regulation, pathogen defense and Ca2+ signaling. Among these regulation pathways, sequences involved in light regulation owns the highest proportion. Furthermore, a Venn diagram was generated to compare functions of genes regulated by AtbZIP16, AtbZIP17 and HY5. Results revealed that, although these three transcription factors all belong to the AtbZIP family and are predicted to be regulated by some similar physiological regulation process (e.g. light), they still possess distict biological functions in plant development. Further studies are thus required to put these transcription factors into their shared and unique biological context. Taken together, the results of this experiment not only indicated light is a key regulation factor for AtbZIP16 and AtbZIP17, but also showed the function of AtbZIPs could be diverse.

人潮、海潮、歷史的浪濤讓高雄這條生命母河—愛河，敘述了許多膾炙人口的故事，記錄著許多人的回憶。五年級上鄉土課程時，在教材中有一章是講『生命母河』—愛河，整章介紹愛河的源頭、流經區域、兩岸的設施及整治的方法。而現在愛河經過了整治及美化綠化後，我們心裡想知道愛河的水到底有多乾淨了，所以我們對整個下游感潮河段做深入的研究，我們研究和調查的結果是：一、愛河主流全長約12 公里，流經高雄市區約11 公里。二、愛河為了整治已經在治平橋設立閘門來截流，讓上游的水不再流到下游。三、愛河從民國57 年開始整治，一直到現在還沒有停止。四、愛河從高雄橋(出海口)一直到九如橋河段水質很乾淨，九如橋到治平橋水質較髒。五、感潮河段很適合魚類生存，水面也可以看到白鷺鷥。六、潮汐確實對愛河的水質有影響，而且對愛河的自淨也有幫助。七、整個下游段兩岸已建設得非常漂亮，尤其是以親水的觀念去設計了木棧道、親水台階、親水平台，河中又有許多魚，是一個非常適合漫步、垂釣、休閒的好地方。