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簡易變色筆

一群勇於探索的學生,想要以酸鹼變色的原理製作出一組簡易可行的變色筆。探索的過程中,一路從指示劑的材料、筆桿的製作方式、提升書寫效果一直延伸到了防腐的方法。在這為期八個月的探索中,師生們無不絞盡腦汁想辦法使變色筆變的更好,其中細緻如熬煮指示劑的方法,都是有待克服的難題。經由不斷嘗試,找出了八種指示劑裝填進自創的吸管筆。以鹼性溶液變色,又以酸性溶液復原。並試著以簡單的材料防止指示劑的腐壞。師生們無不期待研究成果能普及化,讓看過的人也能利用隨手可得的生活素材,簡易地做出一組實用的變色筆。

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Night Vision Goggles

Hadley has create a system that enables you to see in the dark, night?vision goggles. The military version usually retail for thousands of dollars, but Hadley’s solution costs considerably less. And they really work! He has even attached close?fit rubber swimming fittings to the eyepieces to ensure you can’t see a thing without the help of the electronics. The key feature of this project is the way Hadley has used his imagination to access and modify existing technology and explore ways of ‘hacking’ these device to obtain useful component system that he can integrate to make a fully functioning product. In doing this he has encountered many unsuitable combinations, but has persisted to reach an extraordinarily effective endpoint. The whole process, which has taken over a year from initial idea to result is a demonstration of tenacity and ingenuity.

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氯酸鈉( NaClO3 )結晶之研究

第四章時,學到結晶法可以分開溶質與溶劑,但是分離效果如何呢?並沒有進一步討論。第八章討論晶體形狀時,知道不同的離子化合物,有不同的形狀,例如食鹽NaCI是正立方體,但是此形狀是否一成不變?形狀是否會受其他離子的干擾呢?向老師請教的結果發現學長們已在進行實驗,正因高三功課繁忙而暫停,於是我們欣然繼續接下棒子。

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Mechanism of the subcellular localization of the actin binding protein adducin

Adducin蛋白在細胞骨架的調節上扮演著重要的角色。然而,近來有許多研究指出,骨架蛋白也會出現在細胞核並參與轉錄調控,因此本研究的目的即在探討adducin蛋白是否會進入細胞核中,並參與轉錄調控或具有其他功能。在本研究中,我們將綠色螢光蛋白(GFP)標示的adducin質體DNA,利用轉染技術送入老鼠纖維母細胞株NIH3T3中表現。NIH3T3細胞原本並無adducin蛋白的表現,在共軛焦顯微鏡下觀察,野生型的GFP-adducin蛋白會表現於細胞核與細胞質中。由於adducin蛋白尾端序列攜有可能往核內運輸的訊號,於是將位在此一訊號中的離胺酸718及離胺酸719進行突變,結果發現此一突變株只能在細胞質中表現。此外,蛋白磷酸脢C(protein kinase C)已知能磷酸化adducin蛋白在絲胺酸716及絲胺酸726的位置,於是假設其磷酸化是否與其在細胞內的分布有關。將adducin的絲胺酸726置換成丙胺酸,並不影響其在細胞內的分布。然而將絲胺酸716置換成丙胺酸後,則完全只在細胞核中表現。由於adducin可分布於細胞核,因此我們懷疑adducin蛋白可能與細胞分裂有關,於是本研究利用流式細胞儀分析adducin轉染後NIH3T3細胞的細胞週期。流式細胞儀的分析結果顯示,攜有GFP-adducin或其突變株的細胞與未經轉染的NIH3T3細胞的細胞週期並沒有顯著差異。其次,為了避免因轉染的效率不高而造成統計上的誤差,我們利用顯微鏡追蹤技術觀察攜有GFP-adducin的細胞株,結果顯示攜有adducin突變株的NIH3T3細胞株仍能正常分裂。再者,因為adducin能與細胞骨架中的肌動蛋白結合,所以adducin不同的分布位置可能影響細胞附著與細胞展延的效率。細胞展延試驗的結果顯示,adducin及其突變株對細胞附著與細胞展延的效率並無明顯的影響。本研究的結果證明,adducin的確帶有往核內運輸的訊號,其在細胞質中的分布可能也同時受到絲胺酸716磷酸化的影響。然而adducin的功用似乎與纖維母細胞的分裂與展延無明顯的關聯性。Adducin, an actin binding protein, is known to play an important role in the regulation of the membrane cortical cytoskeleton. More and more evidence indicates that proteins involved in the cytoskeletal regulation could also reside in the nucleus and participate in gene regulation. Thus, the goal of this study is to examine whether adducin is expressed in the nucleus and involved in certain nuclear events. In this study, adducin and its various mutants were fused with green fluorescent protein (GFP) and transfected into mouse NIH3T3 fibroblasts which do not have endogenous adducin for monitoring their subcellular distribution under a laser scanning confocal microscope. The wild-type GFP-adducin was found to be present both in the nucleus and in the cytoplasm. The COOH-tail of adducin contains a motif analogous to the nuclear localization signal (NLS). Mutation of two lysine residues (lysine 718 and lysine 719) located within this motif abolished the nuclear localization of adducin. Moreover, adducin is known to be phosphorylated by protein kinase C at serine 716 and 726. Substitution of adducin serine 726 with alanine had no effect on its subcellular localization. In contrast, substitution of adducin serine 716 with alanine led to only nuclear expression. Nuclear localization of adducin renders it possible that adducin may be involved in the regulation of cell division cycle. For cell cycle analysis, flow cytometry was applied. The results of flow cytometry indicated that expression of adducin and its mutants in NIH3T3 fibroblasts did not affect their cell cycle progression. To further examine the effect of adducin on cell division, NIH3T3 cells transiently transfected by adducin were monitored by time lapse video microscopy. The video clearly showed that the cells with GFP-adducin underwent cell division to generate two daughter cells. Since adducin is well known to bind to actin and thereby regulate microfilaments, we wondered that expression of adducin in NIH3T3 cells might affect their adhesion and spreading onto extracellular matrix proteins. The results of cell spreading assays showed that adducin appeared not to affect cell spreading. In conclusion, our results demonstrate that the subcellular distribution of adducin is likely regulated by two signals, one is the nuclear localization signal and the other is the phosphorylation status of the serine 716. However, enforced expression of exogenous adducin in fibroblasts such as NIH3T3 cells does not alter their cell cycle or cell spreading on fibronectin.

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Extreme Mouse Mobile

Objective To design build and run 5 Mouse mobiles – vehicles which use mousetraps as their only source of energy. Targets- • Go up a ramp • Raise a flag • Hit something and set it free • Deliver something • Catapult Details These mouse mobiles are original creations which include some commercially produced parts. They are powered by single spring driven standard wooden mousetraps. They are 5 in number each being used for the respective tasks. Basic structure Each mousetrap consists of a 2 parallel wooden planks which has 2 holes drilled on either sides for the rear and front axles. A rod is used as the axle and CD disks are used as wheels. The mousetrap is attached to the planks. Movement and Locomotion To move the mouse mobile a string is connected to the rear axle. The string runs through 2 pulleys which change the direction of the applied force and are attached to the hammer of the spring. When the vehicle is pulled backwards on the floor tension is created in the string (as the hammer is stretched) which gets stored in the form of spring energy. When the wheels are released on the floor the rear axle starts rotating hence propelling the mouse mobile forward. Completion of objectives 1) To go up a ramp For the required task a high amount of torque would be needed .That will be sufficiently provided by the spring energy. 2) Raise a flag For the required task a rod is placed on the wooden platform. The flag is placed at the bottom of the rod; it is connected to the hammer of the mousetrap using a string. The spring and the rod are placed in the opposite directions. When the hammer is released after being stretched it pulls the string and raises the flag. 3) Deliver something For the required task 2 mouse mobiles are used. A platform of any hard and light material is attached to the rear of a mouse mobile. After reaching the desired location, when the hammer of the primary spring is fully relaxed it hits the catch of another stationary mouse mobile. The hammer of the respective catch is connected to the platform using a string. Upon activation the hammer is released, the platform is pulled with extremely high speed and the materials on the platform, due to their moment of inertia fall down. 4) Hit something and set it free. For the required task 2 mouse mobiles are used. A small mousetrap is attached to the front of the mouse mobile which is in contact to the required object. The other mouse mobile on reaching the above vehicle hits its catch using its hammer. The hammer of the stationary mouse mobile is released and the object is hit and set free. 5) Catapult For the required task 2 mouse mobiles are used. A mousetrap is attached to the front of stationary mouse mobile which is attached to a strainer and the object to be catapulted is placed inside it .Another mouse mobile approaches is and hits the catch hence activating the hammer and the object is catapulted.

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Cu++與綠豆芽中維他命C含量關係的研究

綠豆芽中維他命C 合量甚多,其生長不受季節地域的影響,所以在蔬菜、水葉生長受限制的地域或季節,綠豆芽是一種維他命 C的主要來源,根據綠豆芽中維他命 C含量測定的結果顯示,在同樣日照的處理下,Cu++及發芽日數為影響綠豆芽中維他命 C含量的變因,由實驗結果顯示1.在適常﹝Cu ++﹞ 處理下可提高維他命 C的含量2.孵綠豆芽以第六天維他命 C 含量最高。

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我們的觀察---硼砂對生物的影響

我們從報紙、雜誌及華視“跳動七十二”節目報導中知道,目前許多食品均以硼砂、硼酸做為防腐劑,如果長期攝取,將導致硼的蓄積,便人體引起紅斑、嘔吐、腹瀉、循環系統之破壞、休克、昏迷、腦驚厥等病症,因此引起我們的好奇,硼砂、硼酸真的這麼可怕嗎?

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地磁的測量和磁極庫侖定律之研究

在學習電磁學時,書上有學到磁場的計算,但卻沒有相關實驗,便想自行設計實驗來求得地磁,而在實驗過程中發現,測地磁時可以同時求得磁鐵塊的磁矩和磁極強度,並可進一步驗證兩磁極間的磁力是否符合庫倫定律。

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埃及吹綿介殼蟲的生存之道~蠟泌物與蜜露排除機制探討

校園植栽上有白色綿絮狀生物,經調查和觀察後發現是埃及吹綿介殼蟲(Iceryaaegyptiaca)。一齡若蟲行動活躍找尋適當棲地後行固著生活,以絲狀口器吸食植物汁液。本研究調查埃及吹綿介殼蟲在榕樹及紅雀珊瑚的分佈位置,並比較蟲體各齡期的形態及蠟泌物分佈,結果發現蠟泌物有強疏水性,由長形、弧形和圈圈狀纖維構成,成蟲體側左右各10根蠟絲,蠟絲長度約體長1~2倍,具保護、偽裝、防雨、保護卵和一齡若蟲等功能。一齡和二齡若蟲背部尾端有蠟管,直徑約人類頭髮的1/5-1/6倍,會不斷由基部向前延長,速率約3.5mm/天,蠟管末端會排出葡萄糖含量極高的蜜露,藉蠟管的延長和斷裂可將蜜露排離身體;三齡至成蟲不具蠟管,但全身布滿疏水蠟泌物,蜜露排出後直接沿蠟絲滑離身體。

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湖口台地地形特徵的研究

湖口台地為古淡水河等數條向北方海岸開展之順向河流以石門為頂點所形成的「古石門沖積扇」之一部份。此沖積扇面包括桃園台地、中壢台地、平鎮台地、伯公崗台地、湖口台地等,而以湖口台地為及南,其概略範圍東綠以 20 ~80 公尺高台地崖與大崙面( LT3)相接, 70 公尺左右台地岩與中壢面( LT 3 )相接,西緣以 80 公尺的斷崖接坑子口附近海岸,北緣以約 6O 一 80 公尺的斷層崖接楊梅地溝帶,地溝帶之北即伯公崗與平鎮台地。南緣為關西、新埔間的鳳山溪河床,鳳山溪之南即為飛鳳山丘陵。台地東西延長 14.5 公里,東高而西低,東寬而西窄,形狀恰似一隻大火腿,而積達175.25平方公里。木台地在地形面上屬於紅壤(褚土)緩起伏面,簡稱 L . H 面 ( Lateritic highland)為中新世林口期時因地形、氣候的驟變突然堆積大量紅土與厚礫石層,地形面下可與平鎮、伯公崗相對比。古石門沖積扇各台地大致以扇頂(石門)為最高,而向西或西北海岸方向緩傾,湖口台地扇頂部份銅鑼圈附近卻有一中間較低陷部份,較南、北兩側各低 30 ~40 公尺定右,且這低陷部份由於較低,侵蝕力較弱,原面(300 公尺以上部份)保持十分良好,與其餘各地面有別,如此構成一個使湖口台地更復雜的地形景觀,造成一個值得深思的地形差異,這一低陷部份如何造成的?是否與南北兩側台地而同時形成?有否同屬一個面,都是值得探討的問題。

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讓水均衡一下:定時定量自動灑水器

我們首先從滴水引泉中了解什麼是虹吸現象,探討虹吸現象的操縱、變因;從實驗中觀察水管設置的高低、水流的時間、壓力等各項變因對虹吸現象影響。以及〝不可貪心的魔術杯〞的實驗中,探討倒入杯中的水,如何流出和影響的變因,發現流出水量與彎管在液面下的深度有關,因此可以控制每次流出的水量。我們經由腦力激盪式的討論,又發現它的應用價值,於是我們藉由滴管孔徑大小控制時間間隔,不可貪心魔術杯的彎管控制流量,利用保特瓶設計出第一代的簡易定時定量澆水器。再由實驗記錄和學習單所獲得的經驗,經討論後又設計了更省水、更便利的自動澆水器;結合馬桶、定時開關和抽水馬達等設備,完成第二代的每日定時定量自動澆水器,可應用於定時定量自動捕給冷卻用水或澆花用水,可以簡易完成一週定時定量澆水的工作,若有多餘的水,仍可循環再度使用;特別在種子發芽、小樹苗的培育階段、或冷卻車胎或機械車床等之補給用水。讓澆水工作也能自動化的均衡一下,讓幼苗吸收最適當的水分,長得更健康。

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氣墊船---輕功高手

我們選用了三組高轉速小馬達,一組是馬達車的,一組是氣墊船雛型的,另一組是氣墊船用的。 我們利用氣球來證明反作用力;而積木做成的小車,是從反作用力的運動中,讓車子持續因反作用力而前進。 從實驗中,我們發現雖然馬達車子跑得很快,氣墊船雛型卻跑得不快;於是我們有了分流的概念。我們想到可以讓氣墊船在水中上航行;所以我們把氣墊船的材料改成珍珠板。 原先我們並不知哪一個型式、高度的分流板是最好的,於是我們設計了實驗三。 因為有了划龍舟的印象,所以我們又設計出了方向舵,來控制船的方向。 為了減少接觸面之間的吸附力,我們在船的底部加裝橫桿。 因為原先柔性圍裙的底部開口是全開式的,所以柔性圍裙容易外掀,於是我們才改良柔性圍裙的底部開口成為長條式和多孔式,並將柔性圍裙的長寬比例做不同的比較。 氣墊船在水上比在陸地上的運動情形好,因為在水上多了水的浮力。

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