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「小藻立大功」-應用單細胞微藻製作之重金屬檢測器
重金屬污染中,常見的有錳、銅、鋅、鐵、鎘、鎳等。研究指出,藻類有金屬吸附能力,在環保上,可處理金屬廢水。另外,有些金屬在低劑量會促進藻類生長,過量則會抑制其生長。利用此特性,希望藉金屬濃度與藻數目的高度相關迴歸方程式(R2>0.9),當作生物性的金屬檢測器。實驗中使用單細胞之等邊金藻(Isochrysis galbana)和具相同陰離子之重金屬溶液(ZnSO4、MnSO4、NiSO4、CuSO4)及非重金屬溶液(Na2SO4)進行共培養。結果,求得第7天之NiSO4及CuSO4與等邊金藻數目的高度相關迴歸方程式。利用此NiSO4及CuSO4金屬檢測器,可換算出1g之Na+型陽離子交換樹脂對200mM NiSO4的吸附率為29%,4g為88.9%。而Na+型陽離子交換樹脂對5mM CuSO4的吸附率則達100%。另外,並推測等邊金藻對200mM NiSO4與5mM CuSO4並無吸附力。
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在本次實驗中,我們發現溶液中的帶電離子,會因為離子團的熱運動,和電偶極的庫倫吸引力(electric dipole)的交互作用下,使得電解質溶液的I-V curve(電流-電壓 曲線),具有類似磁滯曲線(Hysteresis curve)的效果,同時我們運用光學干涉的原理,證實此時在電場作用下,離子團會互相順著電場方向作條列鍵結,而加熱實驗也證實,熱運動會使溶液的I-V curve(電流-電壓 曲線)變的不一樣。另一方面,我們也發現,在給予電解質溶液一外加的衝擊電壓時,該溶液的電荷分布,?會因為彼此的互相擠壓以及自由擴散的作用下,而形成一震盪分布,其所顯現出來的,則是電壓的震盪變化。最後由於震盪波紋的變化,在起始時與中段之後有著明顯差異,我們對此變化提出看法和證實,相信內容是精采可期的! In this experiment, with the interaction of the heating action of ionic atmosphere and electric dipole, we find that ionic in the liquor makes the I-V curve in the electrolyte liquor show up with the effect similar to Hysteresis curve. Meanwhile, we practice the principle of interference to prove that at this moment, under the influence of electric field, ionic atmosphere will connect to each other in line following the direction of the electric field. It is also proven that in the heating experiment, heating action will make I-V curve in the liquor different.On the other hand, we also find with extra electric impact, the charge distribution of the liquor will form a oscillation, that is, the changes of the voltage oscillation under the inter-collision and the free spread. We offer our viewpoints and proofs about the obvious changes of oscillation wave in their beginning stage and after their middle stage. We believe that the marvelous content is surely worth of your expectation.
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雞胰臟去氧核醣核酸水解?多型性之探討
去氧核糖核酸水解?,(deoxyribonuclease,簡稱DNase)為一種核酸內切?。目前研究得最透徹的去氧核糖核酸水解?為牛胰臟的DNase I,有四種以上的同功?存在。而牛和雞的DNA序列有極高的相似度,由之前的研究中在等電點焦集電泳法顯示雞胰臟DNase I 具A、B 兩種同功?,本實驗則希望能進一步了解兩種同功?的差異原因。在抽取RNA 後,以RT-PCR 方法合成cDNA,將其以限制?切割,再以大腸桿菌作為載體進行轉殖及繁殖培養,最後將其DNA 定序後進行分析比較。目前已完成第一次定序,但因引子接合過程中有部分位置錯亂造成殖體無法進行表現。另外在此次所得之序列中有發現一個胺基酸的轉變,而其是否為多型性之表現則有待進一步的實驗證實。The most advanced research on deoxyribonuclease in current states is on the bovine pancreatic DNase I, more than four of which isoforms have been found. It is shown that the DNA sequences of chicken and bovine have relatively high similarity. In addition, according to the result of isoelectric focusing from previous researches, chicken pancreatic DNaseI has two isoforms (A and B.) In this research, the author expects to establish more knowledge on the differences on the isoforms and the causes. After receiving the RNA, the RT-PCR is preformed to incorporate the cDNA, which is later cleaved by restriction enzyme and inserted into the plasmid DNA of the E. coli host cell to be cloned. So far a polynucleotide sequence has been deduced from clones of the cDNA, but it cannot be expressed successfully in E.coli due to few random mistaken splicing. There is an alternation of one amino acid, and whether it is the actual state of isoform or not still requires further proof.
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有二系統A和B,A中一開始有2k個物體,,B中有0個物體。在一個單位時間內,兩系統可以互相轉移最多一個物體。當B中物體的個數為 i-1,i∈{1,2,...,k+1},我們稱其為狀態 i,從狀態1﹝初態﹞開始計時,到達狀態 k+1﹝相同態﹞便即刻停止實驗,經過之時間為一隨機變數T,稱之為實驗時間。問當兩個系統的物體數剛好相等時,經過的實驗時間之分佈為何?本文將以上述問題為核心,分別探討不同條件下系統的實驗時間所反映出來的現象,如機率、期望值、變異數等等。
Define two systems, A includes 2k objects, and B has none. They can transfer at most one object from one system to another in a time unit. When the number of objects in B is i-1, i∈{1,2,...,k+1} , we say the system is at state i. As soon as system transfer form state 1 ( initial state ) to state k+1 ( the same state ), the experiment stop. Random variable T, called the experiment time, is the time before stop. What would be the distribution of the experiment time if all systems have the same amount of objects within? This article will focus on the described question and discuss what property the experiment time of the system under various conditions has, such as probability, mean, and variance.
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誰殺死了危險殺手—老祖母的清潔秘方失靈了嗎?之後續研究
台灣氣候潮濕,黴菌及細菌無所不在,對健康及環境均造成污染,如何找出有效又經濟的殺菌方法?本實驗緣自2003 年科展成果後續探討,是不是除了在清潔力之外,能找到可以清潔又可殺菌的肥皂配方,一舉二得,給於更深入的延伸目的及發現,進而能做出更有深度的科學研究。本實驗是以天然素材、具環保、成本低且具殺菌力的肥皂為標的。為了使實驗結果更客觀,我們徵求班上同學的意見,選出外觀顏色最討喜的肥皂。綜合本次研究目的及實驗內容如下:(一) 回鍋油皂基添加哪些添加物可增加殺黴菌能力及殺細菌能力?哪一種效果最佳?(二) 把最好的三種殺菌添加物混合,殺黴菌能力及殺細菌能力如何?與沒有混合的比較?(三) 跟市面上印象中具清潔且殺菌品比較,殺黴菌能力及殺細菌能力如何?成本是否經濟?(四) 肥皂裡加入天然色素,能否改變顏色?顏色是否可變得漂亮?對殺黴菌能力及殺細菌能力有沒有影響?歷經十個月的實驗後,本組實驗得到結論摘要如下:(一) 本實驗所有添加物以「回鍋油+蜂蜜+抹茶皂」(以下簡稱蜂蜜抹茶皂),不論殺黴菌、殺細菌上及使用成本表現最佳。(二) 本實驗自製肥皂,殺細菌能力平均比殺黴菌的能力好,以蜂蜜抹茶皂為例,殺細菌能力是100%,殺黴菌能力是62%。(三) 在殺黴菌能力及殺細菌能力上,比起市面上銷售的清潔殺菌品,本實驗成果(除酒精外)最佳。(四) 給廠商的建議:蜂蜜皂是一個很好的殺菌肥皂(殺黴菌效果50%,殺細菌效果100%),成本低,顏色也很漂亮,但蜂蜜抹茶皂殺黴菌能力及殺細菌能力最佳(殺黴菌效果62%,殺細菌效果100%)。(五) 用黴菌或細菌作實驗不能涵蓋其他菌種的殺菌力,但因其他菌種取得困難或有危險,如SARS 的冠狀病毒、如禽流感病毒,都無法當成小學生的實驗,但我們可用同樣的科學精神,探討未來更多的研究主題,作更有價值的發現。
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本研究探討正方形、正三角形、正六邊形正則鑲嵌格子,無論其是否被塗色,與其相鄰的鑲嵌格子最多僅允許一至數格被塗色的條件,其存在最多塗色格子的數量及存在塗色方式的問題。本研究利用塗色格子位於邊線角落、非角落的邊線、或鑲嵌內部的共用邊數差異、及與塗色格子總數間的限制條件,採用賦值法解析最大塗色格數的上界。接著,利用塗色建構符合解析上界的塗色方式,以數學歸納法推導最大塗色格數的通式,並求證其與解析上界的塗色數量相同,證得確實存在該最多塗色格子數量。研究推廣至n→∞時,各正則鑲嵌塗色面積比率的極限值均收斂至特定數值,且發現當外框邊線效應消失時,以特定週期(鑲嵌層數)累計最大塗色格子數均可表示成數列g(l)=f((l)-f(l-1), h(l+1)=g(l+1)-g(l)≡C2, l∈N, f(0)=C1的形式。
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膜力奇蹟–以蛋殼膜粉吸附重金屬離子與色素之研究
蛋殼膜的主要成份是蛋白質,含有豐富的胺基酸,其結構上的特殊官能基胺基(─NH2 )和羧基(─COOH)對金屬離子具有螯合作用,而且難溶於水。實驗結果顯示蛋殼膜粉對於帶正電的金屬離子(包括H+離子)以及食用色素,皆能有良好的吸附效果。此外我們發現H+離子與金屬離子存在著競爭關係,在較酸的環境下,即使銅離子濃度比氫離子濃度大100倍時,蛋殼膜粉仍優先吸附氫離子。蛋殼膜粉對離子的螯合能力比較為H+>Cu2+>Co2+>Ni2+。以蛋殼膜粉螯合Cu2+離子而言,最小偵測極限可達10-4M。蛋殼膜粉螯合金屬離子與吸附色素的過程為一個可逆反應,利用較高濃度的酸性水溶液,使反應逆向進行達到再生,再生的蛋殼膜粉仍然具有螯合銅離子與吸附色素的能力。
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Few plants can grow in the place where Wedelia trilobata grows. In the following experiment, the leaves ans stalks(of the plants from Wedelia trilobata) are extracted for experimental purpose .The data and records of the experiment have shown that Wedelia trilo ata indeed refain the growth of plants such as mung bean, teeding seed, yeast’s respiration and the photosynthesis. We use the function of dieing the polyphenols to know that the Wedelia trilobata can produce Allelochemicals called as the polyphemols The mesophycell produce the polyphemols from chloroplast of cortex,the polyphemols transport to root and release from root exudation. The plant will absorb polyphemols of the soil, and will affect the growth of the plant. If we can separate and purify the Allelochemicals, we can make it as natural herbicideb. 南美蟛蜞菊所在之處,少有其他植物,本實驗採南美蟛蜞菊植栽區之土壤與南美蟛蜞菊莖或葉之萃取液進行相剋作用之研究,結果顯示南美蟛蜞菊確實會抑制小麥與綠豆種子萌發及幼苗生長,並抑制酵母菌的呼吸作用及植物光合作用中之光反應。 利用多酚類染色方法得知南美蟛蜞菊產生的相剋化合物為多酚類,且該多酚類由葉肉細胞與莖內皮層中具葉綠體之細胞產生,經韌皮部輸送至根,再由根經泌濾作用釋出。土壤中的多酚類會被植物吸收,進而影響植物的生長與發育。 若能將該相剋化合物---多酚類分離與純化,或可作為具選擇性之天然除草劑。
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This study was to explore the nature of two basic constitutes of the regular pentagon,With these two constitutes, the regular pentagon could be multiplied into any times in size. We used four multiplication methodsto show how the regular pentagon enlarge and to verify that the enlarged regular pentagons derived from computer did exist. By integrating these four multiplication rules, we were able to arrange regular pentagon of any length of side, and evidenced the equation was ( If m,n is the number of A,B of a regular pentagon respectively ) When we tried to verify if any regular pentagon could be constituted by other smaller regular pentagons, we found that it was un-dividable only if the length of pentagon side were (the number of A, B were the 2n and 2n-1 item of Lucas Sequence), otherwise, any regular pentagon is able to be constituted by other smaller regular pentagons. The divided forms could be multiple. We also found that any pentagon could be divided by two successive un-dividable pentagons, which is called “standard division rule”. We expected to derive all kinds of division by analysis of two successive un-dividable pentagons in standard division rule.
這個研究起源於一個拼圖玩具:利用兩種黃金三角形排出指定大小的正五邊形。我們的研究動機是:一、 假如無限量供應A 和B,能夠拼出哪些邊長的正五邊形?二、 哪些拼好的正五邊形不能拆成一些較小的正五邊形?我們將研究的主要結果分述如下:
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本研究在不改變傳統汽車電動後視鏡機械結構下,設計出一可自動調整後視鏡視角的智慧型汽車後視鏡。本研究首先以真值表研究電動後視鏡的機電特性,並藉以設計出一可以與原先人工控制後視鏡並存之驅動電路。微電腦單晶片被使用來控制後視鏡之轉向時間及轉向條件。後視鏡之轉角偵測乃利用方向盤帶動齒輪,藉由齒輪間的傳動並利用位置編碼器將轉角資訊轉換成為電訊號,並將訊號傳送至單晶片來控制後視鏡之轉向時間。研究結果顯示,一可隨方向盤轉動而自動改變後視鏡視角之汽車後視鏡確能減少開車時視角的因轉向而減少的問題。
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以阻抗匹配調整太陽能最大功率輸出之研究
In recent years, the price of the oil keeps rising continuously. As a result, the prices of the commodities are rising, too. But what does this mean? This situation stands for the resources on are becoming more and more valuable. A few months ago, I read a Weekly Business Magazine and a Scientist Magazine. They both pointed out that the resources such as oil would disappear after fifty years, and that was a horrible phenomenon. Since almost everything in our modern lives are related to oil, like automobile, motorcycle and air plane. They all need oil for its ingredient. Even plastic bag plays a part in the products of it. I really cannot imagine what it would be like if we don’t have oil after fifty years. Owing to the green house effect is becoming more severe, there are many substitution resources found, such as hydraulic power, wind power and solar energy…etc. What we’re discussing in our topic is how to enhance the power of solar energy, because for now, we all know that the solar module is very expensive and it cost a great deal of money just only one square meter, but the price of module converted from sunlight or heat energy can’t be higher. Therefore, the work is mainly to design and carry out a solar max power point track. 近年來,油價不斷的上漲,連帶著民生的物價漲幅也是越來越可觀,但…這意味著什麼 呢??這所代表的是地球上的資源可以說是越來越珍貴了,前陣子曾經看過商業週刊、科學人 雜誌…他們紛紛所指石油這種能源可能在五十年後就消失殆盡,這個可是非常可怕的結果。 鑑於溫室效應愈趨嚴重,許多的替代能源紛紛出籠,像是風力、水力和太陽能等等。我們這 次的主題是在討論如何提高太陽能效率,現在的太陽能模組我們都曉得非常的昂貴,但是由 太陽轉化成電能的效率卻是低的可以。所以本作品主要目的在於設計與實現一個太陽能最大 功率追蹤器。
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