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線上教學資源

科學大聲公廣播劇08~自製溫度計

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同「鋅」協力 —— 以醇解法探討金屬鋅催化解聚 PET之反應

聚對苯二甲酸乙二酯 (PET) 是一種常見的合成高分子材料,其被應用的範圍相當廣泛,如產品包裝、手提袋等,然而隨著人類的過度使用,越來越多的 PET 產品被發現有回收不當的現象。 文獻指出,醇解法為一具有反應條件簡單、反應過程溫和及較不會產生多餘副產物的解聚 PET 方法,且醇解法能使用非均相觸媒,具有易於分離觸媒的優勢,具工業化潛力。本研究嘗試利用廢電池中的鋅作為催化劑用以解聚 PET 成單體對苯二甲酸乙二酯(BHET),並深入討論影響反應進行的因素與發展應用。利用廢電池中的鋅作為催化劑不但成本低廉,也更能符合本研究的核心「綠色化學」。 本研究已透過最適化條件搜尋,發現能在催化劑使用量為 0.2 克、反應時間 4 小時、反應溫度 180 度時,解聚 5 克的 PET,有最佳效果之 BHET 產率 75.8% 和 PET 轉化率 93.2%。根據最適化反應條件,並比較不同種的鋅化合物做為催化劑,發現廢電池中的鋅作為催化劑催化反應,具有較佳的轉化率與產率。

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信標和微波雷達技術在健康照護之研究

本作品包含三個部份:1.利用微波雷達模組感測輸出都卜勒信號頻率與被檢測目標移動速度成正比關係,只須動態取樣和判斷都卜勒信號頻率變化就能實現一種實用的非穿戴式跌倒偵測器。2.將大型室內空間劃分為多個子空間,整合信標藍牙技術、智慧手機和LoRa無線界面,即可在遠端對整戶或任一子空間執行快速目標搜索。3.利用信標藍牙、微波雷達和毫米波雷達等技術,使傳統醫療院所護士推車具備解鎖啟動保護、目標位置檢測與方向修正、自動跟隨、用手輕壓觸控控制盒表面即能執行“手把手”工作模式和前進無段變速等多項創新功能。在不同實驗條件下,分別對完成後之不同實驗模型進行功能量測、記錄和分析,實驗結果顯示本作品確實可以滿足預期設計目標。

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炭為觀止—生物炭改良土壤成效初探

我們準備了砂質土、黏質土並加入不同比例的生物炭,想要了解在乾旱(0.5倍水)、正常給水和淹水(3倍水)的情況下,兩種不同的土壤對作物生長狀況有什麼影響。透過基礎實驗一~五,我們發現生物炭具有吸水、保水、排水等效果;而在主要實驗一~六結果中,我們發現除了正常給水情境下,在缺水及淹水情境下,加入生物炭也能發揮其效能,會影響作物成長高度,使得作物成長高度因有加入生物炭而成長效果較佳。本次實驗我們只選用風倒木生物炭來探討以及蘿蔓萵苣作為種植的樣本,未來也許可以嘗試種植不同的作物,及拉大生物炭比例的加入對不同農作物的生長有什麼影響,或取用不同金門農作廢棄物製作生物炭,探討對農作物或土壤改良的影響情形。

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Expectations for extension of cell life and next generation anticancer drugs by using secondary metabolites of actinomycetes

Inhibitory effects of the secondary metabolite of actinomycete were examined on cell cycle of the yeasts of S. pombe and S. cerevisiae. The secondary metabolite was obtained from cultivation of the actinomycete isolated from the soil of Owakudani in Hakone, Japan. The fifth fraction of the secondary metabolite by ODS column separation (HK-T5), which was soluble to pure methanol, was used in the present experiments. The HK-T5 brought about the delay of forming colonies of S. pombe for about 11 days compared to that cultivated without the HK-T5. The delay of the colony formation was longer for the S. pombe cultivated with more amount of the HK-T5. The cultivation with HK-T5 also brought about the extension of the lifespan of the S. pombe for more than 10 weeks in a liquidus medium. The cell life recovered the ordinary manner by removal of the HK-T5, meaning that the activities of the HK-T5 is reversible. These facts confirm the suppression of cell cycle, and the delay of cell growth by the HK-T5. These phenomena were similarly observed for S. cerevisiae. Comparison of the action of HK-T5 with hydroxyurea, which is an anticancer drug inhibiting the cell cycle at S phase, clarified that the inhibitory action of HK-T5 worked at the phase earlier than S phase. The combined effects of HK-T5 on the cell cycle were evaluated with triamcinolone acetonide (TA), or aspirin, the former of which is a drug synchronizing cancer cells in S phase, and the latter keeping human cells in G1/G0 phases. The combined use of HK-T5 with TA synchronized the cells at the phase slightly proceeding from G1 to S phase without toxicity. On the other hand, the combined use with aspirin made the inhibitory effect of HK-T5 inactive. Hence, the HK-T5 is attractive as a drug for the extension of cell lifespan, and anticancer therapy.

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點滴監控器

新冠肺炎疫情猛然爆發,全球各地都陷入恐慌,在關注疫情同時,我們也注意到國內醫護人力嚴重不足的警訊,所以我們嘗試利用本次的實驗作品,期許能以科技的便利,增進護理工作的效能,進而降低護理人員的工作負擔並提升醫療品質。 以往注射點滴時,護理師需親自監控或委請病患家屬幫忙留意點滴液位,於是我們嘗試使用樹莓派連接攝像頭來讀取點滴液位並顯示在樹莓派和網頁上,當液位低於我們所設定的數值時,LED燈和蜂鳴器會發出警示,同時IFTTT會發送簡訊通知護理師,達成以科技功能提升護理人力效率,並改善人力不足的問題。

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從空中看地面—用算樹實驗探討行道樹遮陽效果

本研究運用多元的科技方法研究行道樹之規劃。先利用實際測量了解有無樹蔭對地表溫度之差異,再以NDVI數據資料探討六都種樹情形。算樹實驗過程以小型空拍機拍攝市區常見的樹種行道樹,運用ImageJ及IC_Measure程式軟體分析、計算樹冠、樹冠幅、樹冠幅蔭等面積,探討樹蔭大小和遮陽的關聯性,發現樟樹能提供較多樹蔭達降溫效果,是栽種行道樹的最佳選擇。最後自製道具實際模擬,得到最佳植樹高度公式為LH+Wtanθ(單位:m),其中W:要遮住的路寬、LH:樹木枝下高、θ:當地12點太陽仰角,藉「算樹實驗」可配合各地區的太陽仰角角度,計算不同時段的樹蔭面積,系統化規劃各地最適宜植樹高度,如在北回歸線位置6m寬的道路旁建議最低樹高約為12m。

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多邊形的剖分圖形數量之探討

從參考資料[1]可知,將凸n+2邊形利用n-1條不相交的對角線剖分成n個三角形的圖形數量即為卡特蘭數Cn。而我利用不相交的對角線把n+2邊形剖分成數個多邊形和三角形的組合,並從此類的剖分圖形與三角剖分圖形之關聯,進而由卡特蘭數的一般式推導出此類剖分圖形數量的一般式。在本研究中可得,若到把n+2邊形剖分成一個k+2邊形和多個三角形的圖形數量是(2n-k+1 n+1) ;把n+2邊形剖分成一個k+2邊形、一個m+2邊形和多個三角形的圖形數量,當m≠k,數量為n+2/2(2n-k-m+2 n+2) ,當m=k時,數量為n+2/2(2n-2k+2 n+2) ;把n+2邊形剖分成一個k1+2邊形、一個k2+2邊形、一個k3+2邊形、和n-k1-k2-k3 個三角形的剖分圖形,當k1,k2,k3兩兩相異時,數量為(n+2)(n+3)(2n-k1-k2-k3+3 n+3) ;把n+2邊形剖分成一個K1+2邊形、一個K2+2邊形、一個K3+2邊形、一個K4+2邊形和n-K1-k2-k3-k4個三角形的剖分圖形當k1,k2,k3,k4兩兩相異,數量為(n+2)(n+3)(n+4)(2n-k1-k2-k3-k4+4 n=4)。並猜測若k1,k2,...,ki兩兩相異時,把n+2邊形剖分成一個k1+2邊形、一個k2+2邊形、…、一個ki+2邊形、和n-Σkj 個三角形的剖分圖形數量為(n+i)!/(n+1)!(2n-Σkj+i n+i) 。

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茶葉冷知識之渾不守色~探討紅茶的乳化現象

紅茶,是日常生活中常見而且是我們愛喝的飲料之一,偶然發現紅茶表面漂浮著一層酷似油的白色物質,覺得很好奇。後來拜訪茶葉專家、查詢資料也設計實驗,展開一系列有關紅茶乳化的探究與實驗,得到相關研究結果及建議如下: 1.會產生乳化現象的紅茶其實含有較豐富的成份,有機紅茶是不錯的選擇。 2.運用100℃或40℃之RO水以1:50或1:75的比例泡茶。 3.建議泡茶時可以多次拉動茶包,使茶葉在水中跳躍,釋出茶中物質。 4.維持茶溫或是讓茶溫急速降低,較不容易產生乳化現象。 5.從電解實驗得知:負極的茶水區比較有反應,我們推論茶葉中的兒茶素趨於和金屬離子產生反應,所以我們推論喝茶能有助於人體排毒(茶中的兒茶素能吸附人體中的不需要的金屬離子)。

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PVA unveiled the actual role of starch in the Briggs-Rauscher reaction

The Briggs Rauscher reaction (BR reaction) is one of the famous oscillating reactions; the aqueous mixture of KIO3, H2SO4, H2O2, C3H4O4, MnSO4, and starch exhibit color change between yellow and blue-purple repeatedly. The blue-purple color formation is due to the iodine test reaction caused by inclusions of polyiodides such as I3- and I5- in the helical structure of starch. Therefore, starch has been regarded as only an indicator in the BR reaction. But our seniors have found that the oscillation did not last without starch. They hypothesized that starch’s linear helical framework is necessary to elongate the lifetime of the oscillating reaction. If this hypothesis is correct, similar BR-type oscillations must be observed when other polymers with helical structures are used instead of starch. We found the literature which reports that polyvinyl alcohol (PVA) forms a helical structure and indicates the iodine test reaction. In our research, we studied the BR reactions using PVA, with different saponification degrees and viscosities. First, we studied the correlation between the structural features of PVA and the iodine color reaction by spectroscopic approach, exhibiting that PVA with low saponification form helical structures and show the iodine color reactions, which gives red color solutions. Second, we found that additions of the helical-structured PVA to the reaction solution instead of starch induces the BR-type oscillating reactions, while PVA without helical structure induces only a few numbers of oscillations. This is the world-first example of the oscillating reaction using PVA. The oscillation that lasted for 6 minutes with 23 oscillations was almost the same as that of the general BR reaction using starch. We concluded that the polymers with helical structures are intrinsic to elongate the lifetime of the BR reaction. Furthermore, we found that the addition of K3[Fe(CN)6], which has a high redox activity, in the reaction solution with PVA drastically elongated the lifetime (50 min) and increased the numbers of the oscillations (nearly 100 times). This result suggests that the oxidation-reduction reactions by the ferricyanide ion promotes the redox process of iodine and iodide ions.

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Expectations for extension of cell life and next generation anticancer drugs by using secondary metabolites of actinomycetes

Inhibitory effects of the secondary metabolite of actinomycete were examined on cell cycle of the yeasts of S. pombe and S. cerevisiae. The secondary metabolite was obtained from cultivation of the actinomycete isolated from the soil of Owakudani in Hakone, Japan. The fifth fraction of the secondary metabolite by ODS column separation (HK-T5), which was soluble to pure methanol, was used in the present experiments. The HK-T5 brought about the delay of forming colonies of S. pombe for about 11 days compared to that cultivated without the HK-T5. The delay of the colony formation was longer for the S. pombe cultivated with more amount of the HK-T5. The cultivation with HK-T5 also brought about the extension of the lifespan of the S. pombe for more than 10 weeks in a liquidus medium. The cell life recovered the ordinary manner by removal of the HK-T5, meaning that the activities of the HK-T5 is reversible. These facts confirm the suppression of cell cycle, and the delay of cell growth by the HK-T5. These phenomena were similarly observed for S. cerevisiae. Comparison of the action of HK-T5 with hydroxyurea, which is an anticancer drug inhibiting the cell cycle at S phase, clarified that the inhibitory action of HK-T5 worked at the phase earlier than S phase. The combined effects of HK-T5 on the cell cycle were evaluated with triamcinolone acetonide (TA), or aspirin, the former of which is a drug synchronizing cancer cells in S phase, and the latter keeping human cells in G1/G0 phases. The combined use of HK-T5 with TA synchronized the cells at the phase slightly proceeding from G1 to S phase without toxicity. On the other hand, the combined use with aspirin made the inhibitory effect of HK-T5 inactive. Hence, the HK-T5 is attractive as a drug for the extension of cell lifespan, and anticancer therapy.

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Consensus-based Machine Learning Model in the Scoring for Hepatic Steatosis Severity

研究目的:脂肪肝的盛行率在全世界的現代化國家都在增加。病理醫師在評估脂肪肝的嚴重等級時,常缺乏參考意見以減少差異。許多地區也缺乏病理醫師。本研究旨在建立一個有效評估脂肪肝嚴重程度的病理組織學人工智慧模型。研究過程:本研究經由臺中榮民總醫院人體研究倫理審查委員會審核通過,篩選後取得病理資料庫中適合的肝臟組織切片,由兩位病理醫師獨立為肝組織中脂肪堆積的程度評分。再以病理醫師討論後的共識答案為分級標準,來訓練人工智慧模型。研究結果:在100個樣本的獨立測試中,人工智慧模型和病理專科醫師的評分,有71%完全相同、27%差異只有一個等級、2%差異2個等級、而沒有2個等級以上的差異。結論及應用:我們已初步建立一個可以評估脂肪肝嚴重程度的人工智慧模型。這模型可為將來人工智慧的臨床應用,建立一個良好的基礎。

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