前人研究顯示結網性蜘蛛會因環境中獵物組成的改變而改變其網結構。然而，除了結構之外，構成網的絲的物理性質與蜘蛛網的捕捉效率也應有密切之關連，但目前並無人探討蜘蛛是否會因獵物而調整絲之化學及物理性質。在本研究中，我以人面蜘蛛為材料，探討：(一)其在面對不同類型之獵物時是否調整其網之結構，絲之胺基酸組成和其物理性質；(二)所產生之改變是否能促進不同類型獵物的捕食效率；以及(三)是獵物之養份或振動刺激引起這些改變。我藉著操控獵物的養份及振動刺激將人面蜘蛛分成四個處理組，分別餵以活蟋蟀(C 組)，活蒼蠅(F 組)，死蟋蟀加蒼蠅振動刺激(Cd 組)及死蒼蠅加蟋蟀振動刺激(Fd 組)。在完成食控處理後比較各組間之網結構、絲胺基酸組成、絲物理性質、對不同獵物之留置能力以及振動訊號傳遞能力。實驗結果顯示F 組之蜘蛛所結之網有較大的面積及較小的網目，並有較佳之蒼蠅留置力；然而此結構之改變卻會降低其振動訊號傳遞率。C 組之蜘蛛所結之網有較強之張力及較佳之蟋蟀留置力，而且其所產之曳絲有較高比例之alanine、glycine 及glutamine 並較佳之延伸度及韌度，以因應體型較大之獵物。上述結果顯示人面蜘蛛能調整網的結構、絲的蛋白質組成及物理性質來更有效率的捕捉不同類型的獵物。而由於上述各樣變數在C 及F 組間有顯著差異，而在獵物養份與振動訊號錯置的Cd 與Fd 組間無顯著差異，顯示此二項刺激需同時存在才能使人面蜘蛛在面對不同類型獵物時產生結網行為及絲基因表現之改變。Previous studies showed that orb-weaving spiders may adjust web structures when encountering different types of prey. In addition to web structures, silk physical properties should also be critical in affecting the prey catching efficiency of webs. However, so far no one has studied whether spiders adjust silk chemical and physical properties when encountering different types of prey. In this study, I used the giant wood spider Nephila pilipes to investigate (1) would N. pilipes adjust web structures, silk amino acid composition and silk physical properties when encountering different types of prey (2) would such changes enhance the catching efficiency of different prey and (3) were such changes generated by prey chemical composition or vibration signals? To answer these questions, I manipulated prey chemical composition and vibration signals by assigning N. pilipes into the following four feeding groups: (1) living crickets (group C), (2) living flies (group F), (3) dead cricket but with fly vibration signals (group Cd) and (4) dead flies but with cricket vibration signals (group Fd). After the feeding treatments were completed the following variables were compared between the feeding groups: web structures, silk amino acid composition, silk physical properties, retention time of different prey and vibration signal transmission rate of webs. The results showed that the webs built by spiders fed with living flies exhibited larger area, smaller mesh and longer fly retention time. However, such structural changes also reduced the vibration signal transmission ability of the web. On the other hand, webs built by spiders fed with living crickets were stiffer and retained crickets longer. Moreover, their silks exhibited higher percentages of alanine, glycine, glutamine and consequently higher elasticity and strength to cope with the larger prey. These results demonstrated that spiders can adjust web structures, silk protein composition and silk physical properties to enhance the catching efficiency of different prey. While the aforementioned variables differed significantly between spiders in C and F groups, they did not vary between spiders in Cd and Fd groups, in which groups the prey chemical composition and vibration signals were decoupled. Such result suggested that prey chemical composition and vibration signal were both needed in generating changes in web building behavior and silk gene expression in spiders.

逛夜市時看到有人在射飛鏢，我們突然想到，如果飛鏢射中每個點的機率都相等，那我們是不是可以由射中圓的機率，及外圍的面積來推知圓周率呢？我們先後進行射飛鏢及擲黑豆的實驗發現，實驗過程都不夠隨機而宣告失敗。於是我們以解析幾何的方式將實驗的樣本空間座標化，然後用EXCEL 試算表的亂數製造隨機點，並判斷隨機點的落點。 我們共製造了109個亂數數對，由所得數據分析π的近似值為3.141607304。 我們並進一步實驗發現當短軸的長度固定時，橢圓的面積與長軸的長度成正比。進而推得橢圓面積為 1/4 ×長軸×短軸×π 透過實驗我們也發現，邊長為2r 的正立方體最大圓柱、球體、圓錐體積比為3:2:1。並進一步推得半徑為r 的球體體積為 4/3 r3π 此外，我們思索幾個可以再深入探討的主題： 1. 三角函數Sinθ 的近似值。 2. 對數表的製作。 3. 無理數逼近速度及準確性的探討。

This work presents fundamental research in the field of algebra and the theory of number. The subject of the work is equations of Markov's type (the type of the equations introduced by me earlier which generalizes the classic equation of Markov (x2+y2+z2=3xyz)) of parabolic type with two unknown quantities and their genealogical trees. The following questions appeared when I was working on the equations of Markov's type and constructing genealogical trees to them: are there any other trees besides one for a certain equation; how to find all the genealogical trees for the equation of Markov's type; how to find all the integer solutions with the help of the genealogical trees. This work is devoted to the analysis of these questions. The aim of the work: to create the method of finding all the integer solutions of the equations of Markov's type of parabolic type. The tasks of the work: 1. Carrying out some experimental works to find all the genealogical trees for a concrete equation. 2. Formulating a hypothesis that the curve has a specific part. 3. Research the parabolic type in order to apply the hypothesis to it. 4. To formulate and prove the theorems about the necessary and sufficient conditions of the existence of the genealogical trees of the integer solutions of equations of Markov's type of parabolic type with two unknown quantities. As the result of the work all the tasks have been solved. I worked the method of finding all the integer solutions: : to find all the integer solutions by means finding all genealogical trees of the equations of Markov's type of parabolic type with two unknown quantities you need : 1. To investigate if there any integer solutions‧ a special part of the parabola (if it is a parabola)‧ a special part of the parallel lines (if it is a pair of the parallel lines) 2. To build a genealogical tree from every solution (if they exist). 3. All the integer solutions will be on the constructed trees. I also worked out a computer program which is based on the usage of this method.

A novel technology for detection of trace concentration of ions at ppb level was developed. The detection limit of this method can be reached to 1ppb. The Wheatstone bridge usually be used for determination the concentration of metal ion, the major disadvantage of this technique is the precision too low. Atom absorption spectroscopy is better for monitoring trace metal ion, but the cost of instrument is too expensive. In this study, the oscilloscope was used to modify the Wheatstone bridge Circuit. The difference between the voltages of two connection points of Wheatstone bridge was used to estimate the concentration of ions. The amplification system was used in this method to improve the limit to detect ion from 10 ppm to 10ppb. The parameters of measurement including types of probe, match of Wheatstone bridge resistances, parameters of input signal and electrolysis in solution were also being studied. 本研究主要是發展一個富有創新且經濟的ppb ( parts per billion ; 10-9)級，微濃度金屬離子量策技術，目前本實驗設計已可以達到 ppb的偵測極限，尤其本方法不僅能夠應用於定量，尚可做定性偵測。簡易的溶液濃度技術常使用惠司通電橋的方法，但這個方法的缺點在於精確度較低，而另外常用的儀器是原子吸收光譜儀，卻價格高昂。因此，我們回頭對惠司通電橋的技術作改良和創新技術。首先，最重要的新技術是對示波器的改良。示波器被用來量測惠司通電橋的電位，我們比較電橋上兩特定點的電位來判斷濃度，當兩個點的電位相同時代表溶液電阻和控制電阻相同。但當輸入信號的電壓太強時，示波器上並無法判別細微的電位變化，因此造成量測的精度不高。本組設計新的電路用以放大在高強度訊號的波形細微變化，成功將測量極限由 10 ppm推進到 ppb。另外關於此設計中的各種參數，包括探針類型、惠司通電路的電阻匹配、輸入訊號之強度頻率、電極間電容效應及溶液中可能發生的電解效應等，都有精確的探討。最後的設計結果將是創新且有別於以往的設計概念。

本專題研究之目的，在於運用簡單的原理自製出準確、廉價、適用於低風速範圍的風速計；方法是藉由將一連接轉軸的玻璃片迎風，探討迎風時，玻璃片偏轉夾角和風速之間的關係。實驗過程中同時發展出可以用馬達旋臂的裝置與v=rω的原理，產生穩定可計算風速的環境。研究結果發現：在低風速的環境下，θ 範圍小時玻璃片與地夾角和風速大略成正比的關係，所以我們可以得到近似式” v≒aθ+b”，參數(a,b)依玻璃片而定。另外θ 範圍大時，我們可以使用二次逼近曲線的方程式與內插法來作角度與風速的對應。藉由控制玻璃片的厚度、質量與形狀，我們可以製造出相對應風速範圍的風速計。The purpose of our research is to use easy theorems to make accurate and inexpensive anemometer which is available in low wind velocity. To make such anemometers, we made a piece of glass, which was connected to a axle, to face wind; then, we could figure out the corresponding relation between wind speed and the angle created by the original and the resulting positions of the piece of glass. While we were experimenting, we found that we could make a steady-wind-speed environment by using motors, sticks, and the theorem ”v=rω”. The research showed that the angle created by the original and the resulting positions of the piece of glass and wind speed are in direct proportion in low wind velocity in small θ range. Consequently, we\r can derive the formula ” v≒aθ+b” , for each piece of glass (a,b) can be different. In addition, when in bigger θ range, we can use quadratic approximate equation and interpolation to describe the corresponding relation between the angle and wind speed. By adjusting the thickness, mass , and the shape of the glass, we can make anemometers corresponding to different wind velocity ranges.

Arabidopsis thaliana bZIPs（AtbZIPs）是一群影響層面相當廣泛的轉錄因子，一半以上的 AtbZIPs 基因表現受到光的調控，且近九成的分子機制尚未明瞭，因此探討 AtbZIPs 在植物光調控機制中所扮演的角色將是個有趣的課題。AtbZIP16 與 AtbZIP17 皆被推測會參與光的調控機制，然而迄今少有文獻針對這二個轉錄因子進行更多的研究。因此，我們想藉由細菌單雜合系統（Bacterial one hybrid system）的方法，找出能與 AtbZIP16 與 AtbZIP17轉錄因子結合的 DNA 序列，以瞭解此二轉錄因子調節下游基因表現的分子機制，並探討其在光訊息傳導途徑中所扮演的角色。針對 AtbZIP16 與 AtbZIP17，本實驗分別找到了 7 與10 種可能的結合序列。首先，經由資料庫比對分析，我們發現其序列上帶有的 motifs 功能，主要參與在光調控、環境逆境反應機制、組織發育、賀爾蒙調節、病原菌防禦、鈣離子訊息傳遞等方面，其中又以光調控佔最大的比例。再者，藉由將 motifs 的功能繪製成文氏圖，並與 HY5 （AtbZIP56）做比較，結果顯示，這三個轉錄因子雖同屬於 AtbZIP family，據推測皆受到光的調控，可能參與某些相似的生理調節過程，但都各自具備不同的功能，影響植物體的發育。如此的差異，表示他們有實質上的不同，值得我們更深入的研究。整體而言，本實驗結果除了說明 AtbZIPs 的功能確實廣泛之外，也顯示AtbZIP16 與AtbZIP17 是執行光訊號傳導很重要的調控因子。Arabidopsis thaliana bZIPs (AtbZIPs) is a group of transcription factors affecting a wide range of responses in Arabidopsis. The expression of more than half of the AtbZIPs is regulated by light, and the molecular mechanism for roughly 90% of these AtbZIPs remains unknown. Therefore, the roles AtbZIPs play in Arabidopsis light signal transduction is an interesting topic to pursue. AtbZIP16 and AtbZIP17 have been suggested to participate in the regulation mechanism mediated by light. However, only limited studies for these two transcription factors have been previously performed. For this reason, we intended to determine the DNA-binding sequences for AtbZIP16 and AtbZIP17 via the bacterial one hybrid system to reveal their target binding sites in the promoter region of their downstream genes and to speculate their possible biological function especially in light signal transduction pathway. We have identified 7 and 10 possible recognition sequences for AtbZIP16 and AtbZIP17, respectively. Using motif-finding programs analyses, we found the motifs identified are mainly involved in light and stress signaling, tissue development, hormone regulation, pathogen defense and Ca2+ signaling. Among these regulation pathways, sequences involved in light regulation owns the highest proportion. Furthermore, a Venn diagram was generated to compare functions of genes regulated by AtbZIP16, AtbZIP17 and HY5. Results revealed that, although these three transcription factors all belong to the AtbZIP family and are predicted to be regulated by some similar physiological regulation process (e.g. light), they still possess distict biological functions in plant development. Further studies are thus required to put these transcription factors into their shared and unique biological context. Taken together, the results of this experiment not only indicated light is a key regulation factor for AtbZIP16 and AtbZIP17, but also showed the function of AtbZIPs could be diverse.

My aim was to test the effectiveness of coolboxes under different\r conditions, to use this information to do comparisons using commercially\r available coolboxes and finally to build an effective and cheaper coolbox\r appropriate to Namibia\r The general method used to compare different coolboxes involved\r measuring the rate at which ice melted inside the cool boxes under\r different conditions.

隨著工商業發展，環境污染日益嚴重，對多數生長在這塊土地上的人，無疑造成了非常嚴重的影響。但若想要監控目前環境中的空氣品質，則必須具備專業的訓練，及昂貴的實驗設備，對一般民眾而言，根本就做不到。\r 藉由指標植物對所生長環境的高度敏感性，可以發展出一套純天然且免費的環境污染偵測器，不但方便、免攜帶、無須高級儀器協助、更不需要專業的分析技術。為此，我們以對二氧化硫等空氣污染物極為敏感的地衣作為指標植物，對其進行生態與環境污染關係的一系列觀察，並設計相關的實驗，找出環境污染物對地衣的實際影響，使其能夠實際的應用於日常生活，並可加以推廣，讓人人都可以利用地衣來了解自己所處的環境是否遭受污染，為自己家園的環境優劣把關。\r \r 文摘要 :\r With the development of industry and business , environmental pollutions become more and more serious . Undoubtedly , those pollutions have a great effect on us.\r However , by the means of the indicator plant which is highly sensitive to its environment , we can develop a set of natural and free environmental pollution detectors . In this project , we use lichenes,which are very sensitive to air pollution , to do a series of observations and to find out the influence the pollutants have on lichenes . If we can apply this to our regular lives , everyone can use lichenes to see if their environment is polluted or not .

1.- Purpose of the research This Game is a new education system, where we take as reference a historical event called "The Discovery of America." The objective is to implement a new way of teaching materials using the technology developed in recent decades, where the teacher uses a modern and educational support to keep the mind of the student in ongoing activity; this will allow greater retention which gives result better understanding and more knowledge. The system is designed to model how to teach with the versatility of being a teacher to provide knowledge through a book because it contains the text outlined and summarized the history and examination by the implementation of questions that offer 4 possible answers and answering incorrectly restart the game, it forces the player to pay attention and remember information. 2. Procedures It divided the two-step strategy: 1. Define the game. The player is asked to bring the features of the game. It must recognize three: a) The technical aspects, allowing the appropriation of technological language and sometimes the understanding of the technology used to play is essential for future acquisitions; b) the commercial tab: identify which company developed the game, age or classification suggested, price, where purchased, country of origin, etc.., a review of the business tab allows to point out habits, and c) a description of the game, review of the plot, characters, history, objectives, modality, gender (role, simulation, strategy, battle, etc..) duration, etc.. This category allows the recognition of recreational preferences of the subject. 2. Thinking the game. Before, during or after the game the subject must think what you do to win or how the game unfolds. Contrary to what seems, while playing one thinks and sometimes thinks that performing other tasks and this time it is the player or the viewer understand the way it works and identify the physical or mental is used to play, trying to understand what I do but most of all how I do it, it is metacognition. 3. Data This prototype is structured in RSS: stands for Ruby Scripting System: System with Ruby Scripting for games. Object-Oriented programming "OOP" is a model used by the most programming languages, that lets you use objects and their relationships to program what will be the final application. In order to expand its use were included languages spoken in America and expanded platform for different operating systems. 4. Conclusions With this fun game is a simple way to learn the story in time, the environment and the circumstances where the player is the student of our system that shapes the teacher, book review, making it a modern and practical way to teach with the advantage of keeping the mind active during the use of our game, this allows the continued interest in the student normally lost in conventional classes.

思考公車車門開關時在地面上掃過的區域形狀與面積時，發現其中變動的直線為過定點與座標軸圍成三角形面積最小的直線，我們很好奇，這類圍成最小面積的直線更進一步的包絡現象，所以就動手去嘗試做研究。\r \r The research is done out of the curiosity we had when we pondered over the area and the shape a bus door sweeps on the floor when it opens or closes, and thus to discover the graph of the varying line which forms the smallest area so that we attempt to do research on such envelopment phenomena.

在傳統的解剖學分類上，台灣地區樹蛙科(Rhacopboridae) 共有台北樹蛙(Rhacophorus taipeionus)、莫氏樹蚌(R.multrechtis ) 、裴翠樹蛙(R.sp.)、白領樹蛙(Polypedates leuco leucomystax)、褐樹蛙（ R.robostus ）日本樹蛙 ( R.japonicus)、艾氏樹蛙 （ R . eifingeri ）等七種，其中除白頜樹蛙屬於 Polypedates 屬外，其餘六種均屬Rhacophorus 屬（呂， 1982 ）。這七種樹 蛙除裴翠樹蛙族群較小外，其餘六種在台灣都有不小的族群分佈，裴翠樹蛙是師大呂光洋老師實驗室民國七十年五月間新發現，依其外部形態與傳統解剖學分類，將其哲時歸屬於 RhacoPhorus 屬， 很可能是世界新種；本實驗目的之一，在研究裴翠樹蛙基本生化資料，進而對其在分類上位置之確定提供參考”國內關於無尾類(Anu-ra)的生化方面研究甚少，國外有關蟾蜍科（ Bufonidae ）、樹蟾科（ Hylidae ）、赤蛙科 ( Raniidae ）等的生化分析論文發表相當多，但卻很少看到樹蛙科（Rhacophoridae ）的文獻，內此樹蛙科的研究有共必要；本實驗目的之二，在研究台灣本區鄉土的樹蛙 生化資料。根據 Matthews ( 1975 )對蟾蛛( Bufo )的研究， Lew-ontin and Hubby ( 1966 )對果蠅（ Drosophila ）的研究 , He-dgecock and Ayala ( 1974 )對山椒魚（ Salamand ridae ）的研 究， Adest ( 1977 )對蜥蜴（ Iquanidae ）的研究都顯示酯酶（ Esterase ）是多型性現象（ Polymorphism ）最明顯的一種酶，因此取酯酶做此研究甚為適合。

安非他命的濫用在台灣是非常嚴重的公眾健康及社會問題。安非他命會導致一連串的行為異常，包括在中腦紋狀體內釋放多巴胺及阻止多巴胺回收來增加使用者的活動力。由於安非他命會對腦細胞造成傷害，本研究的目的為探討低劑量、無立即毒性之安非他命(類似於人類使用習慣)長期施打下，是否會對C57BL6 小鼠大腦紋狀體內的蛋白質表現有影響。因此利用西方點墨法分析施打低劑量安非他命(2 到6 mg/kg) 約一星期之後，C57BL6 小鼠的大腦紋狀體中一些重要蛋白質(包括腺.酸受體A2A-R、第五亞型腺.酸環化.AC5、caspase-8 及PARP) 的表現是否有改變。實驗結果顯示，低劑量安非他命處理對這些蛋白質的表現並沒有明顯的差異。但利用二維電泳法可看到有少許蛋白質，在經過安非他命處理下有顯著的差別，如KIAA0193 homolog 、GOS-28、gammacrystallin A、malate dehydrogenase 和phosphoglycerate mutase isozyme B (PGAM-B)。這些蛋白質中，malate dehydrogenase 和PGAM-B 與代謝和產生ATP 有關，但前者是增加的，而後者減少，推測安非他命會影響神經細胞的能量代謝，因此長期施打安非他命對紋狀體造成的影響值得進一步探討。;The wide spreading use of amphetamine (AMPH) in Taiwan has become a serious public health and social problem. AMPH evokes a series of behavior abnormality including enhanced locomotor behavior by releasing dopamine and inhibiting dopamine-uptake in the striatum. Since AMPH is known to cause brain damage, the purpose of this study is to investigate the expression of several important proteins in the striatum of C57BL6 mice after chronic treatment with low and non-toxic dosages of AMPH (mimicking the common usage pattern of AMPH addict). C57BL6 mice were daily IP-injected with various dosages of AMPH (0 to 6 mg/kg) for one week. Expression levels of A2A adenosine receptor (A2A-R), adenylyl cyclase type V (AC5), caspase-8 and PARP in the striatum were analyzed by Western blotting analysis. Most proteins examined were not affected by this 1-week AMPH treatment. By the aid of two-dimensional gel electrophoresis, expressions of a few striatal proteins (such as KIAA0193 homolog, GOS-28, gammacrystallin A, malate dehydrogenase and phosphoglycerate mutase isozyme B (PGAM-B) in AMPH-treated mice were altered. Note that malate dehydrogenase and PGAM-B are two enzymes involved in energy metabolism and ATP generation. Interestingly, the former was increased and while the latter was decreased in AMPH-treated mice. Collectively AMPH may affect the energy metabolism in neuronal cells. These results suggest that the injury induced by long-term AMPH exposure warrants our further concerns and investigation.