Adducin蛋白在細胞骨架的調節上扮演著重要的角色。然而，近來有許多研究指出，骨架蛋白也會出現在細胞核並參與轉錄調控，因此本研究的目的即在探討adducin蛋白是否會進入細胞核中，並參與轉錄調控或具有其他功能。在本研究中，我們將綠色螢光蛋白(GFP)標示的adducin質體DNA，利用轉染技術送入老鼠纖維母細胞株NIH3T3中表現。NIH3T3細胞原本並無adducin蛋白的表現，在共軛焦顯微鏡下觀察，野生型的GFP-adducin蛋白會表現於細胞核與細胞質中。由於adducin蛋白尾端序列攜有可能往核內運輸的訊號，於是將位在此一訊號中的離胺酸718及離胺酸719進行突變，結果發現此一突變株只能在細胞質中表現。此外，蛋白磷酸脢C(protein kinase C)已知能磷酸化adducin蛋白在絲胺酸716及絲胺酸726的位置，於是假設其磷酸化是否與其在細胞內的分布有關。將adducin的絲胺酸726置換成丙胺酸，並不影響其在細胞內的分布。然而將絲胺酸716置換成丙胺酸後，則完全只在細胞核中表現。由於adducin可分布於細胞核，因此我們懷疑adducin蛋白可能與細胞分裂有關，於是本研究利用流式細胞儀分析adducin轉染後NIH3T3細胞的細胞週期。流式細胞儀的分析結果顯示，攜有GFP-adducin或其突變株的細胞與未經轉染的NIH3T3細胞的細胞週期並沒有顯著差異。其次，為了避免因轉染的效率不高而造成統計上的誤差，我們利用顯微鏡追蹤技術觀察攜有GFP-adducin的細胞株，結果顯示攜有adducin突變株的NIH3T3細胞株仍能正常分裂。再者，因為adducin能與細胞骨架中的肌動蛋白結合，所以adducin不同的分布位置可能影響細胞附著與細胞展延的效率。細胞展延試驗的結果顯示，adducin及其突變株對細胞附著與細胞展延的效率並無明顯的影響。本研究的結果證明，adducin的確帶有往核內運輸的訊號，其在細胞質中的分布可能也同時受到絲胺酸716磷酸化的影響。然而adducin的功用似乎與纖維母細胞的分裂與展延無明顯的關聯性。Adducin, an actin binding protein, is known to play an important role in the regulation of the membrane cortical cytoskeleton. More and more evidence indicates that proteins involved in the cytoskeletal regulation could also reside in the nucleus and participate in gene regulation. Thus, the goal of this study is to examine whether adducin is expressed in the nucleus and involved in certain nuclear events. In this study, adducin and its various mutants were fused with green fluorescent protein (GFP) and transfected into mouse NIH3T3 fibroblasts which do not have endogenous adducin for monitoring their subcellular distribution under a laser scanning confocal microscope. The wild-type GFP-adducin was found to be present both in the nucleus and in the cytoplasm. The COOH-tail of adducin contains a motif analogous to the nuclear localization signal (NLS). Mutation of two lysine residues (lysine 718 and lysine 719) located within this motif abolished the nuclear localization of adducin. Moreover, adducin is known to be phosphorylated by protein kinase C at serine 716 and 726. Substitution of adducin serine 726 with alanine had no effect on its subcellular localization. In contrast, substitution of adducin serine 716 with alanine led to only nuclear expression. Nuclear localization of adducin renders it possible that adducin may be involved in the regulation of cell division cycle. For cell cycle analysis, flow cytometry was applied. The results of flow cytometry indicated that expression of adducin and its mutants in NIH3T3 fibroblasts did not affect their cell cycle progression. To further examine the effect of adducin on cell division, NIH3T3 cells transiently transfected by adducin were monitored by time lapse video microscopy. The video clearly showed that the cells with GFP-adducin underwent cell division to generate two daughter cells. Since adducin is well known to bind to actin and thereby regulate microfilaments, we wondered that expression of adducin in NIH3T3 cells might affect their adhesion and spreading onto extracellular matrix proteins. The results of cell spreading assays showed that adducin appeared not to affect cell spreading. In conclusion, our results demonstrate that the subcellular distribution of adducin is likely regulated by two signals, one is the nuclear localization signal and the other is the phosphorylation status of the serine 716. However, enforced expression of exogenous adducin in fibroblasts such as NIH3T3 cells does not alter their cell cycle or cell spreading on fibronectin.

流量計在實驗室與工業領域裡是重要的儀器，如今已經有數十種依不同物理原理而發展出來的型式，可以配合多變的環境需求與測量條件而使用。然而，各種流量計所適用的範圍備受侷限。本研究主要目的在發展一種熱線式的渦流流量計，供給氣體之流量量測之用。透過自行製作儀器與設備：熱線測速儀(包括探針、探棒及電子處理器)和渦旋產生器(管道中含一三角形截面之鈍體，當流體通過時，在後方尾流產生週期性渦旋逸放)。由於熱線測速儀擁有偵測流體運動時高頻動態變化的能力(約為20000 Hz 以內)，因此結合熱線測速儀與渦旋產生器，經適當的設計與調校，可以測得在不同流體流速時渦旋產生器的三角截面鈍體後方渦旋逸放的頻率。由於渦旋產生器的截面面積為固定值，因此可以從而計算出流量與渦旋逸放頻率的關係。經由嚴格的校準與驗證步驟，本研究的結果顯示自製的熱線測速儀擁有極佳的渦旋頻率偵測能力，所量測到的校準曲線顯示渦旋產生器的三角形截面柱所產生的渦旋逸放頻率與流量成線性關係。為了降低誤差，建議在0 ~ 40 CMM 之量測範圍內分成三條方程式來代表不同範圍內的校準曲線，最大誤差僅在5%以下。若需使用在不同的流量範圍時，僅需改變渦流產生器和幾何尺寸，以使渦旋逸放頻率適合於熱線測速儀的動態響應範圍即可。倘若商品化之後，可以實際應用於風扇流量量測、引擎進氣埠流量的測量等等應用。熱線測速儀本身也可作為風速計，適用於各種場合之風速量測。Flow meter is a instrument that is vital to the laboratory as well as the industrial related field. Based on different physical principles, tens of models that work in harmony with the diverse environmental demands and measurement conditions are developed to date. However, the application of varied flow meters is still under severe restriction. The purpose of this study is to develop a hot-wire type of vortex shedding flow meter for the use of flow rate measurement. Through the home-made apparatus and device, the hot-wire anemometer (includes probe, stem and electronic processor) and the vortex generator. (duct that contains triangle’s section of the bluff body. When fluid passes through, the wake behind produces periodical vortex shedding.) The ability of hot-wire anemometer when it detects the fluid moving changes of high-frequent movement is within 2000Hz, after appropriate design and adjustment, the combination of hot-wire anemometer and vortex generator may investigate the frequency of different flow rate that generated from the vortex shedding behind the bluff body of triangle section. The section area of vortex generator is constant value, thus it can calculate the relationship of flow rate and the frequency of vortex shedding. By means of strict calibration and test procedure, the results reveal that home-made hot-wire anemometer has excellent ability to detect the frequency of vortex shedding. The calibration curve indicates a linear relationship between the frequency of vortex shedding and flow rate. In order to reduce inaccuracy, it is suggested to classify three formulas to represent the flow rate that ranges from 0 ~ 40 CMM. The greatest inaccuracy is under 5%. When applied to different flow rate range, it only has to change the size of vortex generator only if the response frequency of hot-wire anemometer suit for the range of frequency of vortex generator. After commercialization, it can be applied to measure the flow rate of fans, flow rate of intake valve of engine, etc. Hot-wire anemometer also served as anemometer, which can be applied to wind velocity measurement in any situation.

The highly water-absorbing polymers were prepared by the reaction of acrylic acid as a graft\r monomer and natural starch (eg. rice starch, sticky rice starch or corn starch) as the backbone\r using water hydrogen peroxide and vitamin C as solvent, initiator and catalyst, respectively. The\r reaction was carried out by mixing of starch in distilling water and stirring at 60-70 ℃ for 3\r hours and then cooled to 30℃ Acrylic acid was added and the pH was adjusted to 6-9 by the\r addition of limestone powder. To the stirred mixture were then added hydrogen peroxide (30%\r v/v) and vitamin C and continued stirring for 3 hours until the solution became viscous The\r viscous solution was neutralized with potassium hydroxide (30%v/v) to give the pH for 6.5-7\r and allowed to cool to room temperature overnight. The resulting solution was spread on\r winnowing baskets and allowed to dry under the sun to yield a continuous absorbent polymer\r film. The absorbent polymer film was ground and yield of white powder, The above procedure\r was repeated by using another types of starch. The ability of starch slurry to swell on heating, by\r absorption of water, is it’s most important practical property. Rice starch has very low swelling\r capacity in aqueous media compare with sticky rice starch and corn starch. The capacities of\r water-absorbing polymer were tested by placing polymer powder in the beaker and adding of\r water until it swell and cannot absorb more water. The polymers were found to be an excellent\r water absorbent, which absorbed water about 248, 245 and 167 times in weight of polymer for\r corn starch, sticky rice starch and rice starch, respectively. The water retention was calculated by\r measuring the amount of water releasing after every week for 10 weeks. They have also good\r water retention capacity. The swollen polymers were mixed with soil in ratio 1:3 and the\r increasing water holding capacity of soils which promotes more efficient plant growth in pot were\r test. It was found that the soil could be absorbed water for 10 weeks. It is possible to use highly\r water-absorbing polymers from natural starch in agriculture because they are low in cost and the\r polysaccharide main portion of the product is biodegradable

Two soldiers walk on a checkerboard. They can only walk one step once a time and two directions, front and left, are decided randomly. The gunshot is the column and row where a soldier is located, and one will die if he enters the gunshot area of the other. To treat the probability of winning, we first study the cases of 1×n, 2×n, 3×n, and 4×n rectangles iterately. Then we establish a general form of the probability of winning in a general n×k rectangle by using recurrence technique and generating function, respectively. Finally, we extend to the general n×m×k cuboid case to obtain the first soldier’s probability of winning.在一個長方形的棋盤中，兩士兵行走，每一次只走一步，而且上和左兩個方向是隨機的，射程範圍是所在的此行和此列，而進入他人射程範圍則死亡。探討其獲勝機率，從1×n 、2×n、3×n、4×n 矩形的情形逐步研究，並分別運用遞迴式的技巧及生成函數，導出 n×k 矩形中先走士兵獲勝機率的一般式。更進一步地，我們也獲得了n×m×k 立體空間先走士兵的獲勝機率。

罹患神經母細胞瘤的兒科病人，尤其是罹患stage IVs 神經母細胞瘤者，他們有些伴隨著非常嚴重的貧血，但卻檢測不出神經母細胞瘤已經侵犯骨髓；有時病情來勢洶洶，尤其是腫瘤細胞中已可偵測到N-myc 基因增幅者，診斷時腫瘤細胞可能已在腹腔四處擴散並已侵犯大部分的肝臟。但是，某些這種病患，特別是腫瘤細胞中N-myc 基因沒增幅者，即使在沒有治療的狀況下卻可能有自然恢復的現象，也就是腫瘤細胞會自動消退，但原因仍待進一步的證實與探討。可是，這些病人在其病情最嚴重的時候，骨髓內紅血球母細胞形態上的改變顯示可能與病毒感染有關。但是關於病毒來源的研究，現有的資訊仍然十分有限，其中最重要的是，病毒感染與引發其後天之免疫作用是否有關，更需要深層的研究。因此，為更進一步了解罹患神經母細胞瘤之兒科病人的病毒感染及病毒蛋白表現的作用，我們這次研究的目的在檢驗罹患神經母細胞瘤及貧血之兒科病人與微小病毒B19 (PVB19)、Epstein-Barr Virus (EBV)、腸病毒71 型(EV 71)和巨細胞病毒(CMV)的關係，以及病毒蛋白表現對這些病人的作用與臨床意義。In pediatric patients with neuroblastoma, in particular, those with stage IVs neuroblastoma, sometimes the disease was combined with severe anemia. However, no tumor involvement was detected in the bone marrow. Although some of these patients may have N-myc gene amplification, and the disease could have invaded many abdominal organs, especially liver, interestingly, the disease might regress spontaneously in some of these patients. The medical reason of the spontaneous regression, nonetheless, remains to be determined. It is worth noting that morphological changes of erythroid progenitor cells in the bone marrow have suggested virus infection in these pediatric patients. However, the available information of viral origin is limited. Furthermore, it is possible that the virus infection in these patients could be associated with the revocation of immune responses related to the spontaneous regression of the tumor. In this study we will investigate the relationship of parvovirus B19 (PVB19), Epstein-Barr virus (EBV), enterovirus 71 (EV71) and cytomegalovirus (CMV) with neuroblastoma by PCR in Taiwanese pediatric patients. Moreover, we will study the effect and the clinical significance of viral gene expression as well as N-myc gene amplification in these patients.

The special life cycle of the Nymphoides indica: The veins and petioles of the pleustonic leaves can asexually reproduce adventitious buds. Pleustonic adventitious buds will change the density of the plants and sink into water. When the root of submersible plants attaches to the bottom of the pool, they will extend stems to the water surface and resume the life cycle. Reticulate sigmons and astral sclerenchyma appears in the process of transformation. Astral sclerenchyma merely appear in the species of which its leaves float above the water surface, and Nymphoides sp. are the most obvious. The cell wall thickness of astral sclerenchyma is even. The cells are hollow and full of water, and they will utilize water to change turgor between axes and spines. This is also proved in imitating experiments. The reticulate sigmons are frequently seen among aguatic plants, and the horizontal structures prevent air space from collapsing. These two structures make the thin stems of Nymphoides indica sustain the leaves instead of being crushed by the floating water. 印度莕菜 ( Nymphoides indica )獨特的生長週期：浮水型葉的葉脈和葉柄可以無性繁殖不定芽，浮水型的不定芽會改變植株的密度而沉入水中，當沉水型植株的根系在池底固著後，便會以莖延伸出水面，再次展開浮水型葉的成長週期。轉變過程中會出現網狀的橫膈和星狀厚壁細胞。星狀厚壁細胞僅出現於葉片需由水中浮出水面生長的植物種類，而莕菜屬植物更明顯。星狀厚壁細胞其細胞壁厚度均勻，細胞中空且充滿水分，會利用水分在中軸與突起的棘間移動來改變膨壓變化，在模擬的實驗中也得到證實。網狀的橫膈在水生植物中是較常見到的構造，水平方向的支撐可以讓氣室不會塌陷，兩種構造的作用下使得印度莕菜細長的莖能夠支撐葉片，不會被流水沖斷。

本研究主要是探討翼端小翼對飛機飛行的影響，翼端小翼在現在不少的飛機上都有這種設計，假設小翼可以阻止飛機機翼末端的氣流上旋，進而增加升力與推力，讓飛機能提高飛行時的效率，為了驗證這個假設，因此製作了簡易風洞對小翼的升力與阻力進行定性和定量的探討。升力與阻力的定性定量探討是經由10 組主機翼與五個小翼組合，共有2000 次的測試記錄，再轉化成折線圖予以比較研究，而得到一個穩定性數值結果。這測試實驗的數值結果顯示：小翼可以增加升力，但是也會增加阻力，為了降低阻力，小翼的剖面最好是有弧度。The purpose of this research is to find out the effect resulted from the winglet of the plane to the flight. Many a winglet is nowadays designed for the airplane. Assumes the winglet can stop the air of the tail section of the airplane to revolve up, further increase the force of the raise and the push, and uplift the efficiency of the flight. In order to proof this assumption is correct, so makes an easy air hole to do the research of qualitative and quantitative analysis for the force of the raise and resistance. After about 2000 records tested through the combination of ten sets of the main wing and five tiny wings, and transference of curve diagram , we get a steadily value result. This test result appear the first the winglet can increase the force of the raise, and so do the resistance, and the second to have the force of the resistance decreased, it might be better the section of the winglet is not straight but circular.

科展的指導老師在高中發揮了極為重要的角色，他指導學生閱讀前幾屆的重要作品，提出是當的新問題，領導研究與討論，給予類似專家的常識性建議，有些甚至成為學生的共同研究者，我們看到優秀的高中科展作品中，有許多作品背後都有一個優秀的數學老師，以本屆為例如第一名第，第二名。 指導老師在小學更是作品之母。但是小學指導老師的定位，至今仍然非常模糊。在高中由於作品水準很高，教師不易越殂代庖，但是在國小，國中指導老師很容易不由自主的陷入教的陷阱，以至於許多人認為國小指導老師就是把材料成功地教給學生。其實這是極為錯誤的看法。例如初小組肥皂泡膜角度與經濟網絡的作品，我們注意四一Ｏ四到如果教師讓學生先實測各種泡膜的角度，在得出角度都是120度的經驗歸納結果。相信兒童對於自人界的現象的數學性會有更大的驚奇，如今教師先告訴學生120度的事實，再去檢查，這種「驚奇」的驚人浪費，令我們感到非常可惜。事實上，現在已經有少數小學老師已經覺察到，如果指導老師可以更早成立研究群，更有耐心，更願意傾聽兒童在做什麼，只要找出適當的問題，兒童一樣可以有探索研究的活動。只是這種活動還需要數學教育的學者做更進一步的研究已歸納其研究活動的特質，可?科展評審的參考。當然初小和高小的差異很大。至於適當的問題雖然難找，但也不是沒有。例如初小四一Ｏ一從數字方塊到數字八卦以及高小正多邊形分割成三角形的分割總數及類型都指出值得效法的新趨勢。 國中生正處於進入文字符號運作的尷尬期，加上國中各種測試卷的反覆練習繁多，剝奪兒童從事研究的時間，一向是科展最弱的一環。這也是本組第二名從缺的原因，其實國中組無強將，反而是指導老師可以大加發揮的地方。事實上，國中生透過科展活動也可以順利甄試昇學，由於名額較少限制，更應多鼓勵學生參與。 本屆和上屆來台的美國ISFF學生作品，都和代數數論有密切關係，事實上，高中生的極限觀念的發展，比代數抽象思考的方展慢。基於此點，建議高中指導老師可以給予學生初等數論以及初等待數數論的標準教科書研讀，增強其運作的層次，代數數論的應用很多，而且也很好，如本屆的問題是說如果「n」事一個個邊皆為有禮數的直角三角形的面積則有此解可以生出無線多個有理直角三角型面積都是n。 科展研究問題的開發一向是科展師生最頭痛的問題。有一本英國空中大學教授MASON所著的「大家來數學地想」可以大大的拓展研究的想像力，數學傳播季刊，Mathematical magazine,Mathematical monthly,Mathematical Gazette,Fibonacci Quarterly,國際或亞代奧林匹克試題,Putnam試題都是傳統方法已外找材料的地方國小可從國中高中取材,但切忌獎什麼微積分代數等名堂。 另外科展老師也必須善於累績資源，以及累積自己的經驗與聲望，以吸引校內的優秀學生參與科展，本屆初小組四一Ｏ依從數字方塊到數字八卦和高中組四四一四方塊數論就是源自同一問題，但兩者的方展缺是大異其趣。四一Ｏ一大做奇偶類型經運作後演化的樹型圖，但四四一四在初步地用符號說明演化後，既一舉跳過此一過程，進入決定性的証明。其中的對比有如GORDON等數學家在不變式的工作和Hilbert一舉用一個存在性定理暫時地結束此一時期的歷史事件。另外科展老師也必須善於累積資源，以及累積自己的經驗與聲望，以吸引校內的優秀學生參與科展。

The larceny is always annoys our family and our society. They usually steal on the day when people working in the office or studding in the school. They always wait for people leave the house, and try the bell until them confirm the owner of the house leaved and nobody at home. Then they will open the door or break the lock of door. So we can find that if any body home or the lock is very hoard to open or to break. Then the thief is always choice to give up. That prove if we con let the thief think the house is some body home. Then it can to avoid larceny. This project is about how to design a system which is used AI technology to be a robot that is like a human. That robot can talk to the thief and can be a special lock when the thieves try to break the lock of door. The goal of this project is to design a robot that will be a door angel. It will let thief thinks that is some people always in the house. So they can not to steal in this house. We use the AI speech recognition & house environment control I/O system to be a robot which is setup in the door. It is like an angel to protect our home and family. And can stop the larceny. 「住宅竊盜犯罪」一直持續困擾著人類社會，住宅竊案最常發生於大白天，小偷常趁著家中成員上班、上學等時機，稍加觀察，再加以測試（按門鈴） ，確定家中無人，下手破壞鎖具侵入搜括；小偷只要遇到非常難開的鎖或認為有人在家就不會進一步的行竊。所以這證明只要讓小偷認為家裡有人，就能防止竊案的發生，而我們的研究目的就是如何在小偷還在徘徊尋找目標時，就要讓他感到這一家不能偷，如何讓他感覺家裡有人，進而打消入侵行竊的意念。我們希望能設計一套系統，利用AI人工智慧語音辨識及家庭環境控制，來建立一個充滿智慧的門神機器人，來事先嚇阻小偷的行動，就像門神一樣，可以預防竊案的發生，並整合大門門鎖內鎖與家電，形成智慧型的門神機器人，來保佑我們的家庭，也讓竊盜率降低，作為竊盜犯罪防治的利器。

Miracles in life originate from daily life itself. What can be imagined can really be achieved. Hong Kong, as an international city, is small in area and densely populated. To utilize space, tall buildings inevitably emerge. Taking elevators has therefore become the daily routine of everyone of us. However, problems abound with the present elevator system. You have probably gone through the dreadful episode of waiting long for an elevator to come, and passage being interrupted by unreasonably frequent stops. Besides, it wastes space, since each elevator tube can only accommodate one elevator. This is why our Free-D Elevator System will revolutionize the present one. It consists of a large cylindrical tube, which can be divided into several smaller, imaginary sector-shaped tubes. In each small tube a large number of elevators move vertically by magnetic levitation. The elevators can also rotate from one tube to another, thereby avoiding collision. This innovative design will substantially increase the number of available elevators for given space. Most importantly, it is highly feasible.

The paper represents the results of study of remains of mammalians which inhabited the Adycha-River basin (Verkhoyansky Region) during the Pleistocene Epoch. All studied materials belong to the Museum of Paleontology and Ethnography of the Adychinskaya Secondary School. For a long time, People have been finding ancient animal bones on the Adycha-River banks, and since 1955, the teacher, Bozhedonov Nikolai Gerasimovich, has been organizing exploring local lore expeditions for school children purposed for mammoth fauna investigation and material collection for the school museum. In this research collected material was classified, measurements of the Pleistocene Period animal bones were made. On the basis of it we came to conclusion that during the Pleistocene Epoch, very rare species of animals inhabited the Adycha-River basin. To our opinion, the Adycha-River basin is a unique site for research activity. Comparative measurements of the Pleistocene Periods animal bones were made. Of flu work we know measurements of the Early and Middle Pleistocene Period elephant’s bones which inhabited the Adycha-River basin. Elephants inhabited with mammoth. To our opinion, they didn’t yield to size to mammoth. In that time the Adycha-River basin, was been inhabited by camelopard, bison and Soergelia.\r Museum has 2 skull’s fragments and ovibos fronts part of horny stream. Geological age which is Early Pleistocene or late Pleistocene. These bones concern to Praeovibos. Distance between horns is more 100 mm. If we compare with ovibos skull of the Late Pleistocene we see that distance between horns is longer for the 10 time. We couldn’t found such comparisons at scientist’s works. It results that the more distance between horns is longer the more ancient, and skull’s sizes are much less.\r 7-8 km lower of Betenkes, in disclosing “?????? ???????” and sand bank “??????”, was been founded 3 crag’s part of skull of Soergelia, metacarpal bone and 2 skull’s fragments with horns part. On the Adycha-River basin was bun founded above all quantity of soergelias remains.\r In the Pleistocene Period inhabited a lot of horses. Which remains he have now. Our horse’s height in crest is from 142 to 156 sm. And what about of horses of the Early Pleistocene, it is more than 230 sm.\r Molar teeth of the Early Pleistocene Epoch horse are more for the 2.5 times than of contemporaneous analog.

Hadley has create a system that enables you to see in the dark, night?vision goggles. The military version usually retail for thousands of dollars, but Hadley’s solution costs considerably less. And they really work! He has even attached close?fit rubber swimming fittings to the eyepieces to ensure you can’t see a thing without the help of the electronics. The key feature of this project is the way Hadley has used his imagination to access and modify existing technology and explore ways of ‘hacking’ these device to obtain useful component system that he can integrate to make a fully functioning product. In doing this he has encountered many unsuitable combinations, but has persisted to reach an extraordinarily effective endpoint. The whole process, which has taken over a year from initial idea to result is a demonstration of tenacity and ingenuity.