摘要或動機

我們得知從高雄糖廠及堆肥中篩出的嗜高溫好氧菌Geobacillus thermoleovorans T4，是本種細菌首先被報導具有纖維素分解能力的菌株，但目前僅止於學術研究階段，尚未實際應用於廢棄纖維素的分解上。本研究以廢棄農作物纖維素取代學術研究所用的羧甲基纖維素(Carboxymethyl Cellulose，CMC) ，將T4菌株置於稻稈與米糠培養基內，進行分解效能比較，再利用酵母菌的發酵作用進一步將葡萄糖轉化為酒精。本研究發現，在60℃的環境中，活化的T4 菌株可在CMC、稻稈及米糠培養基中生長繁殖且發揮其分解纖維素為葡萄糖的能力，尤其在米糠培養基中的分解效果最快也最好。此外，將生產的葡萄糖加入酵母菌之後，初步發現也能成功地進行發酵作用產生酒精，生質酒精的產出指日可待！;The Research on Using Geobacillus thermoleovorans T4 to Turn the Deserted Cellulose in Agriculture into glucose in Order to Produce Bio-Renewable Energy It has been found that Geobacillus thermoleovorans T4, a thermophilic aerobic bacterial strain isolated from a sugar refinery wastewater （55-60℃）in Kaohsing, Taiwan, can secrete thermostable endocellulase and hydrolyze carboxymethylcellulose （CMC）in some academic research, but it is stillunknown whether T4 hydrolyzes deserted cellulose in Agriculture. The aim of this study is to investigate the best conditions of T4 cellulase activity after mixing with deserted cellulose (such as rice bran and rice straw) by measuring the glucose concentration and bacteria number, and to produce the ethanol by activated yeast. T4 was added rice bran and rice straw medium, and cultured in 60℃ for 10 hours. The number of T4 and the concentration of glucose were measured every two hours. The best conditions were examined by comparing the hydrolyzation efficiency of T4 in different cellulose medium. We observed that T4 grew efficiently in different cellulose medium and hydrolyzed cellulose into glucose, especially in rice bran medium. The yeast also converted glucose into ethanol. Our research may shed light to the development of bio-renewable energy!