臺灣國際科展

本土藥材金銀花的研究與分析

科展類別
臺灣國際科展
屆次
2006年
科別
化學科
學校名稱
臺北市立第一女子高級中學
指導老師
許順吉、江慧玉
作者
史沛青
關鍵字
藥材,金銀花

摘要或動機

本實驗以薄膜色層層析(TLC)、高效能液相層析(HPLC)分析等化學方法,進行金銀花品種差異的鑑識;此外,配合生藥學的顯微鏡檢視,如中藥材組織鏡檢、藥材粉末鏡檢等比對,以期找出辨別金銀花品種的方法。研究至目前為止,由金銀花之薄膜色層分析的Rf值(0.225、0.425、0.7、0.85、0.95)可確認出金銀花藥材,並得知金銀花藥材中皆含有綠原酸的成分;以高效能液相層析的圖譜與成分峰的積分面積可用來判別金銀花的品種,並從質譜分析瞭解成分含量;進行生藥學的藥材粉末組織鏡檢,發現無法作為金銀花藥材的分類憑藉。未來將持續延伸實驗,朝向中藥奈米化與一般粉末在藥效、成分上差異的比較,並進行金銀花萃取液的抗菌作用試驗,瞭解不同品種之金銀花藥材在藥理作用的異同,接續著奈米化藥材的應用與實踐。Using thin layer chromatograph (TLC) and high performance liquid chromatography (HPLC), we can study how to differentiate the species variation of honeysuckle; beside, based on the observation of biopharmaceutical microscope, such as comparing the histology of Chinese herbs and its powder, we suggest that we could differentiate the species of honeysuckle. From the present, firstly, we could distinguish the honeysuckle from other herbs by the Rf value of TLC(0.225, 0.425, 0.7, 0.85, 0.95), from which we find that all honeysuckles contain the component of Chlorogenic acid. Secondly, we could tell the species of honeysuckle according to the map of HPLC and the peak area after integration, as well as the integrants of honeysuckle by way of LC-Mass analysis. Thirdly, while studying the histological analysis based on the observation of biopharmaceutical microscope, we found that it shows no difference between all the honeysuckles; thus, it fails to be a scientific method used to distinguish the herb honeysuckle. However, in the biochemical experiments of honeysuckles, we found honeysuckles from different sources and the place of origin shows difference in their antibiotic effect, showing the importance of local medicine. When it comes to my future work, in order to extend my experiments on honeysuckles, I would compare the nano-scale honeysuckle powder with normal-sized one in their clinical effects and components.


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