臺灣國際科展

Phytochemical screening and evaluation of antiangiogenic properties of sapinit (Rubus fraxinifolius) fruit crude extracts

科展類別
臺灣國際科展作品
屆次
2021年
科別
生物化學
學校名稱
Philippine Science High School - Main Campus
指導老師
Ana Victoria R. Lloren
作者
Angelica Vince L. Del Rosario;Vionne Beatrice G. Garcia;Janine Phoebe P. Liwanag
關鍵字
antiangiogenesis、Rubus fraxinifolius、phytochemicals

摘要或動機

Plants are potential low-cost alternatives for cancer treatment. Rubus fraxinifolius or “sapinit” has been found to possess phytochemicals with anti-cancer potential. This project aimed to evaluate the antiangiogenic properties of methanolic R. fraxinifolius fruit crude extracts through the chick chorioallantoic membrane (CAM) assay. Through phytochemical screening, leucoanthocyanins, phenols, and tannins were detected. For the CAM assay, 10, 20, and 30 μg/μL extracts, distilled water, methanol, and retinoic acid were applied on 60 ten-day-old chicken eggs. The CAM photographs were analyzed using ImageJ Software. The mean percent inhibitions (MPI) of total length and vascular density from both analyses were subjected to One-Way Analysis of Variance (ANOVA). The ANOVA for the MPI of total length, followed by a Tukey post hoc test, show that only retinoic acid treatment has significantly higher MPI (p = 0.0010). Meanwhile, the results for the MPI of vascular density show no significant differences between all groups (p = 0.1630). It is possible that the concentrations used in the study may not be the concentrations needed to achieve optimal antiangiogenesis. The results may also be due to the absence of phytochemicals that exhibit significant antiangiogenic properties such as alkaloids. Lower concentrations and isolated phytochemicals may also be tested.


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