臺灣國際科展

Discovery, Cloning and Recombinant Expression of a Coral Peptide with anti-Bacteria activity

科展類別
臺灣國際科展作品
屆次
2018年
科別
醫學與健康科學
得獎情形
一等獎
學校名稱
PuiChing Middle school, Macao
作者
Ieong Sin U; Chan Chi Kio

摘要或動機

Inflammatory Bowel Disease (IBD) is a prevalent disease of the West which pathogenesis is driven by a combination interaction between bacteria and inflammatory cells. In this study, two Kazal domain peptide from Palythoa Caribaeorum were identified. They were found to exhibit serine protease inhibitory, anti-bacterial effects and low toxicity, making them ideal candidates for IBD treatment due to their ability to inhibit inflammatory cell migration and bacterial load. We amplified their coding DNA sequences via PCR and ligated the resulting PCR product into pGEX-4T3 vector. The recombinant plasmid was verified by sequencing, and restriction digest before being transformed into competent E.coli cells. Following transformation, we induced target peptides expression by IPTG to confirmed successful transformation and peptide production. Selected transformed bacterial colonies were expanded in LB broth before mixing with glycerol and frozen in -80°C freezer to complete the process of cell bank production.


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Discovery, Cloning and Recombinant Expression of a Coral Peptide with anti-Bacteria activity 471 KB Adobe Reader(Pdf)檔案