The Characterization of Human Epidermal Stem Cells

Stanford University
Stefan Vitorovic
Human Epidermal Stem Cells


The role of Notch signaling in the regulation of growth and differentiation of epithelial stem cells is poorly understood. While specific markers for epidermal stem cells have not yet been identified, members the Notch signaling pathway have been reported to be differentially expressed in the human epidermis. This study sought to demonstrate the presence and distribution of Notch and its ligands, Delta and Jagged, in human keratinocytes, and thereby characterize this subpopulation. Human neonatal foreskin samples were used to obtain isolated epidermal cells. Cells that were shown to be negative for connexin43, a gap junction protein, and positive for keratin14, a basal marker, were classified as presumptive stem cells (PSC). This sorted subpopulation was shown to be small and agranular by flow cytometry analysis. After two weeks in cell culture, PSC revealed a proliferative potential three times greater than non-sorted cells. The PSC exhibited increased expression of Delta and Jagged ligands than the general population. Additionally, RT-PCR confirmed the presence of Jagged and Delta in keratinocytes; however, only Jagged was detected in immunohistochemistry tests. Members of the Notch family were identified by immunohistochemistry in the epithelium and also at the protein- and mRNA-level. The data suggests that variations in the expression of members of the Notch signaling pathway could potentially be used as markers for stem cells of the epithelium; however, further research is necessary to make definitive conclusions, which would provide better insight into Notch regulatory pathways. This understanding could one day allow for the eventual treatment of epithelial damage caused by various skin diseases, injuries, or burns.

「為配合國家發展委員會「推動ODF-CNS15251為政府為文件標準格式實施計畫」,以及 提供使用者有文書軟體選擇的權利,本館檔案下載部分文件將公布ODF開放文件格式, 免費開源軟體可至LibreOffice 下載安裝使用,或依貴慣用的軟體開啟文件。」

檔案名稱 檔案大小 格式
The Characterization of Human Epidermal Stem Cells 12 KB Adobe Reader(Pdf)檔案