臺灣國際科展

日長處理對大豆蛋白質合成的影響

科展類別
臺灣國際科展
屆次
2002年
科別
植物學
學校名稱
高雄市立高雄高級中學
指導老師
王敏男
作者
蘇柏嵐、廖雋安
關鍵字
日長處理,大豆蛋白質

摘要或動機

日照影響花芽分化及生長試植物生理學上的重要課題,根據相關文獻指出,蛋白質類型的變化是影響開化的重要關鍵,大豆是短日照植物,分別以長日照(LD18hr/DAY)和短日照(SD6hr/DAY)處理,抽取葉片中的蛋白質以SDS-PAGE.2D-SDS-PAGE分析,SDS-PAGE的實驗姐果顯示出在分子量25.2 32.9 38.6 20.6 64.0 100.6 kDa處中的蛋白質SD比LD的量多,但是在分子量16.8kDa以下的蛋白質LD含有高濃度而自SD只有一點,我們使用2D-SDS-PAGE做進一步的分析,SD與LD分為三個區塊討論(SD1-3 LD1-3)有許多蛋白自LD1.LD3出現,但在SD1 SD3中未出現,可能有抑制花芽分化的作用,而在SD2中有多個高濃度蛋白出現,可能在花芽分化上扮演重角色,其中編號P1 P2的蛋白質有較明顯的變化,根據NCBI網站上的資料顯示,P1蛋白質與光敏素C有關,而P2蛋白質則沒有找到分子量等電點類似的蛋白質;The effects of photoeriod on floral initation and develoment is an imporant phyiological subject and have studed ve he past 70 years. Accrding to the reference, polypeptide partten plays an important rolein photoperiodic induction. Soybean s a SD(short day)plant. So we planted them with 6-h(short day,SD)and 18-h(long day,LD)photoperiods. Total proteins n leaves of soyban were extracted from the vegetable,SD and LD. And using SDS-PAGE and 2-D eloctrophoresis to analyze. 25.2,32.9,38.6,50.6,64.0,and 100.6kDa polypeptides in SDS-PAGE were always present in leavess at SD but only a little bands f the polypeptides appeared at LD. And many polypeptides whose molecular weight(Mw)is below 16.8 kDa had higher concentration at LD ad Reduced at SD. We found three different areas(SD1~3,LD1~3) and analyze them. Many proteins were found n LD1,LD3 and reduced in SD2,SD5. Possibly, these polypeptides found in LD1 and LD3 play an important role in restraining floral iniiation in soybean. Many proeins had more concentration in SD2. Probably, they play an important role in photoperiodic induction and floral initiation. We found a protein labeled P1 in SD2 and it is with molecular weight(Mw) of 36kDa at isoelectric point(pI) of5.2, and it may be related to cytochromeC. We also found protein labeled P2 in SD2 and it is with molecular weight(Mw) of 40.2kDa at isoelectric point(pI)of 5.4, and we couldn't find any protein that has the similary Mw and pI in potein database about flowering and daylength.


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