臺灣國際科展

Reduction of free radicals and endotoxin by conjugated linoleic acid loaded in-situ synthesized poly

科展類別
臺灣國際科展
屆次
2008年
科別
化學科
學校名稱
臺北市私立開南高級商工職業學校
指導老師
廖顯仁、楊銘乾
作者
吳銘哲、于子捷
關鍵字
聚異丙基丙烯醯胺、共軛亞油酸、氧化壓力、poly(N-isopropyl acrylamide)、conjugated linoleic acid、oxidative stress

摘要或動機

本研究首先利用NaOH 將PAN 薄膜改質形成PAA 水膠膜表面,進一步與異丙胺鍵結形成NIPAAm 結構,以做為藥物傳輸的載體。反應所得之PNIPAAm 薄膜其溫度轉換點為34 °C 其pH 敏感性介於pH 5 至10 之間,實驗進行進一步於34 °C將PNIPAAm 薄膜浸入共軛亞麻油酸(CLA)中,取出後置入4 oC 二次水中,最後測試經改質接枝後PAN 薄膜於膽固醇、三酸甘油酯的吸收效果,並以化學冷光儀分析改質接枝後PAN 薄膜其抗氧化效率。結果顯示改質接枝之PNIPAAm 薄膜於包埋CLA 其對於血液中膽固醇及三酸甘油酯有明顯的吸收效率,此外對血液中的自由基也有明顯較未改質之PAN 薄膜穩定的趨勢。A thin layer of poly(N-isopropyl acrylamide) (pNIPAAm) was synthesized in situ on the surface of hydrolyzed polyacrylonitrile (PAN) membrane. This thin layer exhibited both pH response due to the poly(acrylic acid) moiety and temperature response due to the pNIPAAm moiety. The swelling behavior of the membranes was evaluated under various temperatures and pH. The curve of the swelling ratio for the PAN-NIPAAm showed a lower critical solution temperature (LCST). Then conjugated linoleic acid (CLA) was loaded into the pNIPAAm layer. The effects of CLA on the blood coagulation and oxidative stress were evaluated using human blood. The level of reactive oxygen species (ROS) was measured by chemiluminescence (CL) method to evaluate the oxidative stress. Furthermore, the removal of bacterial endotoxin (lipopolysaccharide, LPS) by CLA-loaded PAN-NIPAAm was measured with ELISA. The results show that the LCST swelling curve was at 37°C. In addition, the swelling ratio increased by 71% when the pH increased from 5 to 10. The concentration of LPS can be reduced by CLA-loaded PAN-NIPAAm 2.1 and 1.2 5 times of that by unmodified PAN and PAN-NIPAAm membranes, respectively. In addition, the level of ROS against CLA-loaded PAN-NIPAAm was reduced significantly than that against unmodified PAN and PAN-NIPAAm. Therefore CLA-loaded PAN-NIPAAm membrane could offer protection for patients against oxidative stress and could also inhibit LPS for clinical applications


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