無機砷與硒對染色體傷害之交互作用

摘要或動機

中文摘要
重金屬對人體傷害的無遠弗屆-人人皆知，根據流行病學的研究指出，長期處在
重金屬含量過高之地區，易造成生理病變。比方說，長期生活在砷含量高的環境中，
易導致肝臟、周邊血管及神經系的損害，各種癌症的發生機率也大為提高。
本實驗以中國倉鼠卵巢細胞（CHO-K1）為材料，利用微核偵測技術(Michronuclei,
MN)及姐妹染色分體互換(Sister chromatid exchanges, SCE)之誘引來探討亞硒酸鈉
(Sodium selenite, NaSeO₂) 及亞砷酸鈉(Sodium arsenite, NaAsO₂) 的交互作用
(interaction)。實驗結果顯示亞硒酸鈉本身不會造成MN 的增加，但讓SCE 頻率增加；
亞砷酸鈉會誘引增加MN 和SCE。亞硒酸鈉前處理不會減少亞砷酸鈉誘引之SCE 和
MN，但可微微降低亞砷酸鈉抑制雙核細胞(Bunucleated cells, BN)的形成，但是不明
顯。本實驗結果未能看出亞硒酸鈉前處理與亞砷酸鈉有明顯的交互作用，但發現亞
硒酸鈉會增加SCE 頻率，因此亞硒酸鈉做為保健食品值得進一步的關切及探討。

英文摘要
It is well-documented that exposure to heavy metals could cause seriously adverse
effects to humans. Epidemiological evidence has shown that illness is frequently observed in
residents living long-term in heavy metal contaminated area. For example, arsenic exposure
was associated with increased incidences of liver diseases, peripheral vascular and
neurological diseases, as well as cancers. In this report we investigated the interaction of
selenite and arsenite on micronuclei (MN) and sister chromatid exchange (SCE) induction in
Chinese hamster ovary-K1 (CHO-K1) cells. The present results demonstrated that sodium
selenite by itself did not induce MN, but it did cause the increase of SCE frequency. Sodium
arsenite significantly induced MN and SCE in CHO-K1 cells. Pretreatment of CHO-K1 cells
with selenite could not reduce MN and SCE frequency induced by arsenite, but slightly
prevent the cells from arsenite-induced inhibition of binucleated cell formation. In the present
study, no significant interaction between sodium selenite pretreatment and sodium arsenite
was observed. However, sodium selenite was unexpectedly found to enhance SCE frequency
in CHO-K1 cells. The application of selenite as health protection agent warrants our concerns
and further investigation.

---

「為配合國家發展委員會「推動ODF-CNS15251為政府為文件標準格式實施計畫」，以及 提供使用者有文書軟體選擇的權利，本館檔案下載部分文件將公布ODF開放文件格式， 免費開源軟體可至LibreOffice 下載安裝使用，或依貴慣用的軟體開啟文件。」

檔案名稱	檔案大小	格式
無機砷與硒對染色體傷害之交互作用	707 KB