人類脂肪幹細胞培養於電漿處理幾丁聚醣薄膜之初步研究

摘要或動機

人類的脂肪幹細胞,取得容易,且來源不涉及倫理問題,是一種理想的城體細胞來源,目前已發現其可分化成骨細胞,軟骨細胞,脂肪細胞,等等,因此深具未來發展的潛力,將可應用於組織工程細胞治療上,而Chitosan(幾丁聚醣)則是一種具生物相容性,生物分解性且無生物毒性的材料,在生醫材料的領域一具相當發展性,在這個實驗中,我們將chitosan薄膜經過氬氣電漿處哩,並測量其與水的接觸角.發現電漿處理時間越長,接觸角越小,表示電漿處理有助於增加chotosan薄膜表面的親水性.細胞在經過電漿處理的薄膜上,其黏附情形一隨處理時間增長,黏著數目越多,且形態愈扁平,表示電漿處理過的chotosan表面有助於細胞的吸附.;Human Processed Lipoaspirate cells (hPLA) can be subtracted easily from lipoaspirate and the source of the cells does not violate the moral and ethic standard. Therefore, it is an ideal source of somatic stem cells. Recently, researches show that the hPLA cells have the ability to differentiate into osteoblast cells, chondrocytes cells, adipose cells, and skeletal muscle cells. In the future, they have great potential in tissue engineering or cell therapy. Chitosan is a biocompatible, biodegradable, and non-toxic material. It is also an advanced material to be used in the biomaterial field. In this study, we treated chitosan film with argon plasma and measured its contact angle with water. The contact angles decreased as the duration of plasma treatment on chitosan increased, indicating that plasma treatment has a positive influence on increasing the hydrophilicity of chitosan film surface. Moreover, the numbers of hPLA cells adhering to chitosan films increased and their morphology became flatter when the durations of plasma treatment on chitosan films prolonged. Hence, the result showed that plasma treatment on chitosan films also promoted the adhesion of hPLA cells on chitosan film surfaces.

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檔案名稱	檔案大小	格式
人類脂肪幹細胞培養於電漿處理幾丁聚醣薄膜之初步研究	1 MB